Low-dose testosterone treatment decreases oxidative damage in TM3 Leydig cells

Yi-Sheng Huang, Tien Ling Liao, Ji Fan Lin, Yi Chia Lin, Shu Yu Lee, Yen Chun Lai, Shu Huei Kao

研究成果: 雜誌貢獻文章

16 引文 (Scopus)

摘要

Testosterone replacement therapy has benefits for aging men and those with hypogonadism. However, the effects of exogenous testosterone on Leydig cells are still unclear and need to be clarified. In this report, we demonstrate that testosterone supplementation can reduce oxidative damage in Leydig cells. The TM3 Leydig cell line was used as an in vitro cell model in this study. Cytoprotective effects were identified with 100-nmol l-1 testosterone treatment, but cytotoxic effects were found with ≥500-nmol l-1 testosterone supplementation. Significantly reduced reactive oxygen species (ROS) generation, lipid peroxide contents and hypoxia induction factor (HIF)-1α stabilization and activation were found with 100-nmol l -1 testosterone treatment. There was a 1.72-fold increase in ROS generation in the 500-nmol l-1 compared to the 100-nmol l -1 testosterone treatment. A 1.58-fold increase in steroidogenic acute regulatory protein (StAR) expression was found in 50-nmol l-1 testosterone-treated cells (P0.01). Chemically induced hypoxia was attenuated by testosterone supplementation. Leydig cells treated with low-dose testosterone supplementation showed cytoprotection by decreasing ROS and lipid peroxides, increasing StAR expression and relieving hypoxia stress as demonstrated by HIF-1α stabilization. Increased oxidative damage was found with ≥500-nmol l-1 testosterone manipulation. The mechanism governing the differential dose effects of testosterone on Leydig cells needs further investigation in order to shed light on testosterone replacement therapy.
原文英語
頁(從 - 到)432-437
頁數6
期刊Asian Journal of Andrology
13
發行號3
DOIs
出版狀態已發佈 - 五月 2011

指紋

Leydig Cells
Testosterone
Reactive Oxygen Species
Lipid Peroxides
Therapeutics
Cytoprotection
Hypogonadism
1-testosterone
Cell Line
Hypoxia

ASJC Scopus subject areas

  • Urology

引用此文

Low-dose testosterone treatment decreases oxidative damage in TM3 Leydig cells. / Huang, Yi-Sheng; Liao, Tien Ling; Lin, Ji Fan; Lin, Yi Chia; Lee, Shu Yu; Lai, Yen Chun; Kao, Shu Huei.

於: Asian Journal of Andrology, 卷 13, 編號 3, 05.2011, p. 432-437.

研究成果: 雜誌貢獻文章

Huang, Y-S, Liao, TL, Lin, JF, Lin, YC, Lee, SY, Lai, YC & Kao, SH 2011, 'Low-dose testosterone treatment decreases oxidative damage in TM3 Leydig cells', Asian Journal of Andrology, 卷 13, 編號 3, 頁 432-437. https://doi.org/10.1038/aja.2010.159
Huang, Yi-Sheng ; Liao, Tien Ling ; Lin, Ji Fan ; Lin, Yi Chia ; Lee, Shu Yu ; Lai, Yen Chun ; Kao, Shu Huei. / Low-dose testosterone treatment decreases oxidative damage in TM3 Leydig cells. 於: Asian Journal of Andrology. 2011 ; 卷 13, 編號 3. 頁 432-437.
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