Low-Density-Lipoprotein (LDL)-bound flavonoids increase the resistance of LDL to oxidation and glycation under pathophysiological concentrations of glucose in vitro

Chi H. Wu, Jer A. Lin, Wen Ching Hsieh, Gow Chin Yen

研究成果: 雜誌貢獻文章同行評審

30 引文 斯高帕斯(Scopus)

摘要

The higher susceptibility of low-density lipoprotein (LDL) to oxidation and glycation in diabetes has been shown to be related to poor glycemic control. The aim of this study was to determine whether LDL-bound flavonoids attenuate high-glucose (HG)-mediated LDL oxidation and glycation. For this purpose, human plasma was preincubated with individual flavonoids for 3 h, followed by sequential ultracentrifugation and extensive dialysis to remove unbound flavonoid samples. Enriched LDL was subsequently isolated and challenged for its resistance to oxidation and glycation. Results showed that glucose (5-30 mM) dose-dependently accelerates copper (Cu2+)-mediated LDL oxidatlve modification. The enrichment of flavonoids such as luteolin, naringenln, and kaempferol significantly increased the resistance of LDL to oxidation and prevented endogenous α-tocopherol consumption caused by HG/Cu2+ (p <0.05). The long-term glycation of LDL, which was measured by advanced glycation endproducts (AGEs)-related fluorescence and boronate affinity chromatography, was found to be inhibited by LDL-bound flavonoids in the following order: rutin > luteolin > quercetin > kaempferol > naringenin > catechin ≈ EC > naringin. Moreover, a solid-phase extraction system with HPLC-diode array detection provided evidence that flavonoids were bound to LDL particles to a certain extent concurrently facilitating the lipoprotein antioxidant and antiglycation activities. In conclusion, this study supports the hypothesis that HG promoted oxidative and glycative modifications of LDL. This is the first study to show that the introduction of flavonoids into LDL particles protects the lipoprotein against glycotoxin-medlated adverse effects.

原文英語
頁(從 - 到)5058-5064
頁數7
期刊Journal of Agricultural and Food Chemistry
57
發行號11
DOIs
出版狀態已發佈 - 六月 10 2009
對外發佈

ASJC Scopus subject areas

  • 農業與生物科學 (全部)
  • 化學 (全部)

指紋

深入研究「Low-Density-Lipoprotein (LDL)-bound flavonoids increase the resistance of LDL to oxidation and glycation under pathophysiological concentrations of glucose in vitro」主題。共同形成了獨特的指紋。

引用此