Long form collapsin response mediator protein-1 (LCRMP-1) expression is associated with clinical outcome and lymph node metastasis in non-small cell lung cancer patients

Szu Hua Pan, Yu Chih Chao, Hsuan Yu Chen, Pei Fang Hung, Pei Ying Lin, Chung Wu Lin, Yih Leong Chang, Chen Tu Wu, Yung Chie Lee, Shuenn Chen Yang, Tse Ming Hong, Pan Chyr Yang

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26 引文 斯高帕斯(Scopus)

摘要

Collapsin response mediator protein (CRMP) family proteins are cytosolic phosphoproteins involved in semaphorin 3A-mediated neuronal cell growth cone collapse and cancer invasion. We identified a novel human isoform of CRMP family proteins named long form CRMP-1 (LCRMP-1), which was different from the known invasion suppressor, CRMP-1, in its molecular weight and the N-terminal exon-1. This study was aimed to elucidate the clinical significance of LCRMP-1 in non-small cell lung cancer (NSCLC) patients. Full-length human LCRMP-1 was cloned from lung adenocarcinoma based on the Expressed Sequence Tags (EST) database. We generated LCRMP-1 specific antibody and subsequent in vitro and in vivo invasion assays showed positive correlations between LCRMP-1 expression and lung cancer cell invasiveness. We further demonstrated that high LCRMP-1 mRNA expressions were associated with poor overall and disease-free survivals (P = 0.004 and 0.006, respectively, log-rank test) in 72 NSCLC patients. The results were confirmed in an independent cohort of 54 NSCLC patients by immunohistochemistry (P = 0.032, log-rank test). The metastatic lymph nodes showed higher LCRMP-1 expressions as compared with the paired primary lung tumors (P = 0.012, McNemar's test). In conclusion, LCRMP-1 was a cancer invasion enhancer that could be a novel prognostic biomarker in NSCLC.

原文英語
頁(從 - 到)93-100
頁數8
期刊Lung Cancer
67
發行號1
DOIs
出版狀態已發佈 - 一月 2010
對外發佈

ASJC Scopus subject areas

  • 腫瘤科
  • 肺和呼吸系統醫學
  • 癌症研究

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