Localization and characterization of an orphan receptor, guanylyl cyclase-G, in mouse testis and sperm

Yen Hua Huang, Chih Chun Wei, Yueh Hsing Su, Bo Tsung Wu, Yi Yun Ciou, Cheng Fen Tu, Trevor G. Cooper, Ching Hei Yeung, Sin Tak Chu, Ming Tzu Tsai, Ruey Bing Yang

研究成果: 雜誌貢獻文章同行評審

14 引文 斯高帕斯(Scopus)


We recently identified a novel testis-enriched receptor guanylyl cyclase (GC) in the mouse, designated mGC-G. To further investigate its protein expression and function, we generated a neutralizing antibody specifically against the extracellular domain of this receptor. RT-PCR and immunohistochemical analyses show that mGC-G is predominantly expressed from round spermatids to spermatozoa in mouse testis at both the mRNA and protein levels. Flow cytometry and confocal immunofluorescence reveal that mGC-G is a cell surface protein restricted to the plasma membrane overlying the acrosome and midpiece of the flagellum in mature sperm. Interestingly, Western blot analysis demonstrates that testicular mGC-G is approximately 180 kDa but is subject to limited proteolysis during epididymal sperm transport, resulting in a smaller fragment tethered on the mature sperm surface. On Fluo-3 cytometrical analysis and computer-assisted sperm assay, we found that serum albumin-induced elevation of sperm intracellular Ca 2+ concentration, protein tyrosine phosphorylation, and progressive motility associated with capacitation are markedly reduced by preincubation of the anti-mGC-G neutralizing antibody. Together, these results indicate that mGC-G is proteolytically modified in mature sperm membrane and suggest that mGC-G-mediated signaling may play a critical role in gamete/reproductive biology.

頁(從 - 到)4792-4800
出版狀態已發佈 - 2006

ASJC Scopus subject areas

  • 內分泌
  • 內分泌學、糖尿病和代謝


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