Local anesthetics induce apoptosis in human breast tumor cells

Yuan Ching Chang, Chien Liang Liu, Ming Jen Chen, Yung Wei Hsu, Shan Na Chen, Chi Hsin Lin, Chin Man Chen, Feng Ming Yang, Meng Chun Hu

研究成果: 雜誌貢獻文章

66 引文 斯高帕斯(Scopus)

摘要

BACKGROUND:: Previous studies have shown that local anesthetics may induce apoptosis in some cell types. In this study, we investigated the apoptotic effects of local anesthetics in human breast tumor cells. METHODS:: Human breast cancer (MCF-7) and mammary epithelial (MCF-10A) cell lines were treated with lidocaine and/or bupivacaine. Cell viability, DNA fragmentation, and annexin V immunofluorescence staining were assessed. The effects on apoptosis-related protein expression were investigated by Western blot analysis. The findings were extended to studies in an in vivo xenograft model. RESULTS:: Treatment of breast tumor cells with lidocaine and bupivacaine resulted in inhibition of cell viability via induction of apoptosis. The effects were more prominent in MCF-7 cells than in MCF-10A cells. Treatment with local anesthetics induced caspase 7, 8, 9, and poly ADP-ribose polymerase cleavage. The cleavage of caspase 7 and poly ADP-ribose polymerase induced by local anesthetics were effectively blocked by caspase inhibitors. Furthermore, treatment of MCF-7 xenografts with local anesthetics resulted in higher expression of cleaved caspase 7 and an increase in terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. CONCLUSION:: Lidocaine and bupivacaine induce apoptosis of breast tumor cells at clinically relevant concentrations. Our results reveal previously unrecognized beneficial actions of local anesthetics and call for further studies to assess the oncologic advantages of their use during breast cancer surgery.
原文英語
頁(從 - 到)116-124
頁數9
期刊Anesthesia and Analgesia
118
發行號1
DOIs
出版狀態已發佈 - 一月 1 2014
對外發佈Yes

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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    Chang, Y. C., Liu, C. L., Chen, M. J., Hsu, Y. W., Chen, S. N., Lin, C. H., Chen, C. M., Yang, F. M., & Hu, M. C. (2014). Local anesthetics induce apoptosis in human breast tumor cells. Anesthesia and Analgesia, 118(1), 116-124. https://doi.org/10.1213/ANE.0b013e3182a94479