Live circulating tumour cells selection on digitized self-assembled cell array (Digi-saca) chip by in-parallel/in-situ image analysis, cell capture, and cultivation

Venkanagouda S. Goudar, Ping Hao Yeh, Shin Yao Wu, Cheng Hsuan Chu, Long Sheng Lu, Chien Hui Yang, Tzeon Jye Chiou, Fan Gang Tseng

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Cancer is one of the major and most deadly diseases of mankind. With present technologies, early detection and prevention of cancer disease is still a major bottleneck. Circulating Tumor Cells (CTC) is one of the cancer biomarkers for the diagnosis of metastasis. However, there is an urgent need to improve the turn around time of CTC detection from a small sample volume in order to promote its clinical implications. This paper highlights Digi-SACA, an automated workflow to capture CTCs from the whole blood on self-assembled cell array chips. The gravity force and lateral driving force based microfluidic chip drive the mononuclear blood cells from 4 ml of samples to form a monolayer without any external fluid control equipment. The subsequent immunostaining and automated image acquisition, image processing, CTC enumeration, and CTC harvest can be completed within 4 h. The sensitivity of Digi-SACA chip is 1 in ten million leukocytes which is very promising for early detection of the CTC. In a small pilot study series, the Digi-SACA chip is able to detect of the mean of 14.4 CTCs from 4 ml of blood in 10 clinical samples from patients with breast cancer, while mean of 17.6 CTCs were measured from the same sample set with IsoFlux™ platform from 4 ml of blood. We have also demonstrated the efficiency of our technique to pick CTC with glass micropipettes for standard cell culture growth up to 11–15 days. Overall, our study represents the liability of the Digi-SACA system in CTCs enumeration, detection, and isolation against the state-of-the-art.

原文英語
文章編號128002
期刊Sensors and Actuators, B: Chemical
316
DOIs
出版狀態已發佈 - 8月 1 2020

ASJC Scopus subject areas

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學

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