Lipid raft assembly and LcK recruitment in TRAIL costimulation mediates NF-κB activation and T cell proliferation

Shih Chia Huang, Huei-Fang Tsai, Horng Tay Tzeng, Hsiu Jung Liao, Ping Ning Hsu

研究成果: 雜誌貢獻文章

16 引文 (Scopus)

摘要

The TNF-related apoptosis-inducing ligand was shown to provide a costimulatory signal that cooperates with the TCR/CD3 complex to induce T cell proliferation and cytokine production. Although a number of signaling pathways were linked to the TCR/CD3 complex, it is not known how these two receptors cooperate to induce T cell activation. In this study, we show that TRAIL-induced costimulation of T cells depends on activation of the NF-κB pathway. TRAIL induced the NF-κB pathway by phosphorylation of inhibitor of κB factor kinase and protein kinase Cθ in conjunction with anti-CD3. Furthermore, we demonstrated that TRAIL costimulation induced phosphorylation of the upstream TCR-proximal tyrosine kinases, Lck and ZAP70. Ligation of the TRAIL by its soluble receptor, DR4-Fc, alone was able to induce the phosphorylation of Lck and ZAP70 and to activate the NF-κB pathway; however, it was insufficient to fully activate T cells to support T cell proliferation. In contrast, TRAIL engagement in conjunction with anti-CD3, but not TRAIL ligation alone, induced lipid raft assembly and recruitment of Lck and PKCθ. These results demonstrate that TRAIL costimulation mediates NF-κB activation and T cell proliferation by lipid raft assembly and recruitment of Lck. Our results suggest that in TRAIL costimulation, lipid raft recruitment of Lck integrates mitogenic NF-κB-dependent signals from the TCR and TRAIL in T lymphocytes.
原文英語
頁(從 - 到)931-939
頁數9
期刊Journal of Immunology
186
發行號2
DOIs
出版狀態已發佈 - 一月 15 2011

指紋

Cell Proliferation
T-Lymphocytes
Lipids
T-Cell Antigen Receptor-CD3 Complex
Phosphorylation
Ligation
TNF-Related Apoptosis-Inducing Ligand
TNF-Related Apoptosis-Inducing Ligand Receptors
Fc Receptors
Protein-Tyrosine Kinases
Cytokines

ASJC Scopus subject areas

  • Immunology

引用此文

Lipid raft assembly and LcK recruitment in TRAIL costimulation mediates NF-κB activation and T cell proliferation. / Huang, Shih Chia; Tsai, Huei-Fang; Tzeng, Horng Tay; Liao, Hsiu Jung; Hsu, Ping Ning.

於: Journal of Immunology, 卷 186, 編號 2, 15.01.2011, p. 931-939.

研究成果: 雜誌貢獻文章

Huang, Shih Chia ; Tsai, Huei-Fang ; Tzeng, Horng Tay ; Liao, Hsiu Jung ; Hsu, Ping Ning. / Lipid raft assembly and LcK recruitment in TRAIL costimulation mediates NF-κB activation and T cell proliferation. 於: Journal of Immunology. 2011 ; 卷 186, 編號 2. 頁 931-939.
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