Line probe assay for rapid detection of mutations in the rpoB gene of Mycobacterium tuberculosis

Yung Ching Liu, Tsi S. Huang, Wen Kuei Huang

研究成果: 雜誌貢獻文章同行評審

8 引文 斯高帕斯(Scopus)

摘要

Detection of mutations in the rpoB gene of Mycobacterium tuberculosis has been reported to be an accurate predictor of rifampin resistance. DNA sequence analysis and screening methods such as single-strand conformation polymorphism analysis and dideoxy fingerprinting are labor-intensive, expensive, or yield results that may prove difficult to interpret. We evaluated the accuracy of a commercial line probe assay for rapid identification and characterization of mutations in the rpoB gene in 72 isolates of M. tuberculosis, including 50 rifampin-resistant and 22 rifampin- susceptible strains. Ten distinct rpoB mutations were identified. Concordances with automated sequencing results and phenotypic rifampin susceptibility testing results were 99% and 93%, respectively. The results demonstrate the line probe assay to be a rapid (as short as 1 day) and informative tool for the early detection and characterization of rpoB mutations associated with rifampin resistance in a clinical laboratory setting.

原文英語
頁(從 - 到)582-585
頁數4
期刊Journal of the Formosan Medical Association = Taiwan yi zhi
98
發行號8
出版狀態已發佈 - 八月 1999
對外發佈Yes

ASJC Scopus subject areas

  • Medicine(all)

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