Leaf extracts of calocedrus formosana (Florin) induce G2/M cell cycle arrest and apoptosis in human bladder cancer cells

Chen Li Cheng, Sheau Yun Yuan, Chi Chen Lin, Shih Lan Hsu, Ya Wen Cheng, Jyh Horng Wu, Chi Rei Yang

研究成果: 雜誌貢獻文章

6 引文 斯高帕斯(Scopus)

摘要

Calocedrus formosana (Florin) bark acetone/ethylacetate extracts are known to exert an antitumor effect on some human cancer cell lines, but the mechanism is yet to be defined. The aim of this study was to determine the effects of Florin leaf methanol extracts on the growth and apoptosis of human bladder cancer cell lines. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed that the growth of these bladder cancer cells was potently inhibited by the Florin leaf extracts. The cell cycle of these extract-treated cells (TCCSUP cells) was arrested at the G2/M phase as determined by flow cytometry. Western blot analysis revealed the increases of cyclin B1 and Cdc2 kinase levels, alone with the decrease of phosphorylated Cdc2 kinase, after treating these cells with the extracts. An immunofluorescence assessment of -tubulin showed decreased levels of polymerized tubulin in treated cells. However, the proteolytic cleavage of poly ADP-ribose polymerase and the activation of caspase-3/-8/-9 were all increased upon treatments of extracts. The concurrent increase of Bax and decrease of Bcl-2 levels indicated that the extracts could induce apoptosis in these treated cells. Taken together, these results suggest that the Florin leaf extracts may be an effective antibladder cancer agent.
原文英語
文章編號380923
期刊Evidence-based Complementary and Alternative Medicine
2011
DOIs
出版狀態已發佈 - 2011
對外發佈Yes

ASJC Scopus subject areas

  • Complementary and alternative medicine

指紋 深入研究「Leaf extracts of calocedrus formosana (Florin) induce G2/M cell cycle arrest and apoptosis in human bladder cancer cells」主題。共同形成了獨特的指紋。

  • 引用此