Large scale chromatographic purification of alpha 1-antitrypsin from human plasma supernatant II + III.

T. Burnouf, J. Constans, A. Clerc, J. Descamps, L. Martinache, M. Goudemand

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3 引文 斯高帕斯(Scopus)

摘要

We describe a large-scale chromatographic method to purify alpha 1-antitrypsin (AAT) from human plasma supernatant II + III. Supernatant II + III was injected onto DEAE sepharose CL 6B to get an albumin-rich fraction as well as a fraction that contained about 45% AAT. This intermediate purity AAT fraction was further purified by size-exclusion chromatography on Sephacryl S-200. The product was heated in solution at 60 degrees C for 10 hours in the presence of a stabilizer to lower the risk of transmission of viral diseases. Purity of the AAT concentrate is over 85%; clinical testing of its safety and efficacy in the treatment of PiZ patients is underway.
原文英語
頁(從 - 到)31-38
頁數8
期刊Developments in Biological Standardization
67
出版狀態已發佈 - 1987
對外發佈

ASJC Scopus subject areas

  • 生物技術

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