Large-pore platelet-rich fibrin with a mg ring to allow mc3t3-e1 preosteoblast migration and to improve osteogenic ability for bone defect repair

Pei Chun Wong, Chen Yun Wang, Jason Shian Ching Jang, Chian Her Lee, Jia Lin Wu

研究成果: 雜誌貢獻文章同行評審

摘要

Platelet-rich fibrin (PRF) is a natural fibrin meshwork material with multiple functions that are suitable for tissue engineering applications. PRF provides a suitable scaffold for critical-size bone defect treatment due to its platelet cytokines and rich growth factors. However, the structure of PRF not only promotes cell attachment but also, due to its density, provides a pool for cell migration into the PRF to facilitate regeneration. In our study, we used repeated freeze drying to enlarge the pores of PRF to engineer large-pore PRF (LPPRF), a type of PRF that has expanded pores for cell migration. Moreover, a biodegradable Mg ring was used to provide stability to bone defects and the release of Mg ions during degradation may enhance osteoconduction and osteoinduction. Our results revealed that cell migration was more extensive when LPPRF was used rather than when PRF was used and that LPPRF retained the growth factors present in PRF. Moreover, the Mg ions released from the Mg ring during degradation significantly enhanced the calcium deposition of MC3T3-E1 preosteoblasts. In the present study, a bone substitute comprising LPPRF combined with a Mg ring was demonstrated to have much potential for critical-size bone defect repair.
原文英語
文章編號4022
期刊International journal of molecular sciences
22
發行號8
DOIs
出版狀態已發佈 - 四月 2 2021

ASJC Scopus subject areas

  • 催化
  • 分子生物學
  • 光譜
  • 電腦科學應用
  • 物理與理論化學
  • 有機化學
  • 無機化學

指紋

深入研究「Large-pore platelet-rich fibrin with a mg ring to allow mc3t3-e1 preosteoblast migration and to improve osteogenic ability for bone defect repair」主題。共同形成了獨特的指紋。

引用此