Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy

Wen Lo, Tsung Jen Wang, Wei Liang Chen, Chiu Mei Hsueh, Shean Jen Chen, Yang Fang Chen, Hsiu Chu Chou, Pi Jung Lin, Fung Rong Hu, Chen Yuan Dong

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2 引文 斯高帕斯(Scopus)

摘要

We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.

原文英語
文章編號036005
期刊Journal of Biomedical Optics
15
發行號3
DOIs
出版狀態已發佈 - 五月 2010

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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