Label-free biosensing of a gene mutation using a silicon nanowire field-effect transistor

Chi Chang Wu, Fu Hsiang Ko, Yuh Shyong Yang, Der Ling Hsia, Bo Syuan Lee, Ting Siang Su

研究成果: 雜誌貢獻文章同行評審

64 引文 斯高帕斯(Scopus)

摘要

We have developed a silicon nanowire field-effect transistor (NWFET) that allows deoxyribonucleic acid (DNA) biosensing. The nanowire (NW) was fabricated on a silicon-on-insulator wafer to provide effective ohmic contact. The NWFET sensor displayed n-channel depletion characteristics. To demonstrate the sensing capacity of the NWFET, we employed the BRAF V599E mutation gene, which correlates to the occurrence of cancers, as the target DNA sequence. The threshold voltage of the NWFET increased when the mutation gene was hybridized with the capture DNA strands on the nanowire, and decreased to the original level after de-hybridization of the gene. The shift in the drain current-gate voltage (I D-V G) curves revealed that the electrical signal had a logarithmic relationship with respect to the concentration of the mutation gene of up to six orders of magnitude, with the detection limit in the sub-femtomolar level. The detection results of mismatched DNA sequences, including one- and five-base-mismatched DNA strands, could be distinguished from complementary DNA gene by this sensor. The excellent electrical results obtained using this label-free NWFET sensor suggest that such devices might be potentially useful tools for biological research and oncogene screening.

原文英語
頁(從 - 到)820-825
頁數6
期刊Biosensors and Bioelectronics
25
發行號4
DOIs
出版狀態已發佈 - 十二月 15 2009
對外發佈Yes

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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