摘要
原文 | 英語 |
---|---|
頁(從 - 到) | 9912-9925 |
頁數 | 14 |
期刊 | Journal of Virology |
卷 | 79 |
發行號 | 15 |
DOIs | |
出版狀態 | 已發佈 - 八月 1 2005 |
對外發佈 | Yes |
指紋
ASJC Scopus subject areas
- Immunology
- Virology
引用此文
Kaposi's sarcoma-associated herpesvirus K-bZIP represses gene transcription via SUMO modification. / Izumiya, Yoshihiro; Ellison, Thomas J.; Yeh, Edward T.H.; Jung, Jae U.; Luciw, Paul A.; Kung, Hsing Jien.
於: Journal of Virology, 卷 79, 編號 15, 01.08.2005, p. 9912-9925.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Kaposi's sarcoma-associated herpesvirus K-bZIP represses gene transcription via SUMO modification
AU - Izumiya, Yoshihiro
AU - Ellison, Thomas J.
AU - Yeh, Edward T.H.
AU - Jung, Jae U.
AU - Luciw, Paul A.
AU - Kung, Hsing Jien
PY - 2005/8/1
Y1 - 2005/8/1
N2 - Kaposi's sarcoma-associated herpesvirus (KSHV) is a human gammaherpesvirus implicated in AIDS-related neoplasms. Previously, we demonstrated that the early lytic gene product K-bZIP is a transcriptional represser that affects a subset of viral gene transcriptions mediated by the viral transactivator K-Rta (Y. Izumiya et al. J. Virol. 77:1441-1451, 2003). Sumoylation has emerged as an important posttranslational modification that affects the location and function of cellular and viral proteins and also plays a significant role in transcriptional repression along with Ubc9, the E2 SUMO conjugation enzyme. Here, we provide evidence that K-bZIP is sumoylated at the lysine 158 residue and associates with Ubc9 both in a cell-free system and in virus-infected BCBL-1 cells. Reporter assays showed that the expression of SUMO-specific protease 1 attenuated the transcriptional repression activity of K-bZIP. The expression of a K-bZIPK158R mutant, which was no longer sumoylated, exhibited the reduced transcriptional repression activity. This indicates that sumoylation plays an important part in the transcriptional repression activity of K-bZIP. Finally, chromatin immunoprecipitation experiments demonstrated that K-bZIP interacts with and recruits Ubc9 to specific KSHV promoters. Thus, our data indicate that K-bZIP is a SUMO adaptor, which recruits Ubc9 to specific viral target promoters, thereby exerting its transcriptional repression activity.
AB - Kaposi's sarcoma-associated herpesvirus (KSHV) is a human gammaherpesvirus implicated in AIDS-related neoplasms. Previously, we demonstrated that the early lytic gene product K-bZIP is a transcriptional represser that affects a subset of viral gene transcriptions mediated by the viral transactivator K-Rta (Y. Izumiya et al. J. Virol. 77:1441-1451, 2003). Sumoylation has emerged as an important posttranslational modification that affects the location and function of cellular and viral proteins and also plays a significant role in transcriptional repression along with Ubc9, the E2 SUMO conjugation enzyme. Here, we provide evidence that K-bZIP is sumoylated at the lysine 158 residue and associates with Ubc9 both in a cell-free system and in virus-infected BCBL-1 cells. Reporter assays showed that the expression of SUMO-specific protease 1 attenuated the transcriptional repression activity of K-bZIP. The expression of a K-bZIPK158R mutant, which was no longer sumoylated, exhibited the reduced transcriptional repression activity. This indicates that sumoylation plays an important part in the transcriptional repression activity of K-bZIP. Finally, chromatin immunoprecipitation experiments demonstrated that K-bZIP interacts with and recruits Ubc9 to specific KSHV promoters. Thus, our data indicate that K-bZIP is a SUMO adaptor, which recruits Ubc9 to specific viral target promoters, thereby exerting its transcriptional repression activity.
UR - http://www.scopus.com/inward/record.url?scp=22544486295&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22544486295&partnerID=8YFLogxK
U2 - 10.1128/JVI.79.15.9912-9925.2005
DO - 10.1128/JVI.79.15.9912-9925.2005
M3 - Article
C2 - 16014952
AN - SCOPUS:22544486295
VL - 79
SP - 9912
EP - 9925
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 15
ER -