Kaposi's sarcoma-associated herpesvirus K-bZIP represses gene transcription via SUMO modification

Yoshihiro Izumiya, Thomas J. Ellison, Edward T.H. Yeh, Jae U. Jung, Paul A. Luciw, Hsing Jien Kung

研究成果: 雜誌貢獻 › 文章

61 引文 (Scopus)

摘要

Kaposi's sarcoma-associated herpesvirus (KSHV) is a human gammaherpesvirus implicated in AIDS-related neoplasms. Previously, we demonstrated that the early lytic gene product K-bZIP is a transcriptional represser that affects a subset of viral gene transcriptions mediated by the viral transactivator K-Rta (Y. Izumiya et al. J. Virol. 77:1441-1451, 2003). Sumoylation has emerged as an important posttranslational modification that affects the location and function of cellular and viral proteins and also plays a significant role in transcriptional repression along with Ubc9, the E2 SUMO conjugation enzyme. Here, we provide evidence that K-bZIP is sumoylated at the lysine 158 residue and associates with Ubc9 both in a cell-free system and in virus-infected BCBL-1 cells. Reporter assays showed that the expression of SUMO-specific protease 1 attenuated the transcriptional repression activity of K-bZIP. The expression of a K-bZIPK158R mutant, which was no longer sumoylated, exhibited the reduced transcriptional repression activity. This indicates that sumoylation plays an important part in the transcriptional repression activity of K-bZIP. Finally, chromatin immunoprecipitation experiments demonstrated that K-bZIP interacts with and recruits Ubc9 to specific KSHV promoters. Thus, our data indicate that K-bZIP is a SUMO adaptor, which recruits Ubc9 to specific viral target promoters, thereby exerting its transcriptional repression activity.
原文英語
頁（從 - 到）9912-9925
頁數14
期刊Journal of Virology
卷79
發行號15
DOIs
出版狀態已發佈 - 八月 1 2005
對外發佈Yes

指紋

Human herpesvirus 8
Sumoylation
Human Herpesvirus 8
transcription (genetics)
promoter regions
cell free system
viral proteins
post-translational modification
Genes
chromatin
lysine
Trans-Activators
proteinases
genes
Viral Genes
Cell-Free System
Chromatin Immunoprecipitation
Viral Proteins
Post Translational Protein Processing
viruses

ASJC Scopus subject areas

  • Immunology
  • Virology

引用此文

Kaposi's sarcoma-associated herpesvirus K-bZIP represses gene transcription via SUMO modification. / Izumiya, Yoshihiro; Ellison, Thomas J.; Yeh, Edward T.H.; Jung, Jae U.; Luciw, Paul A.; Kung, Hsing Jien.

於: Journal of Virology, 卷 79, 編號 15, 01.08.2005, p. 9912-9925.

研究成果: 雜誌貢獻 › 文章

Izumiya, Y, Ellison, TJ, Yeh, ETH, Jung, JU, Luciw, PA & Kung, HJ 2005, 'Kaposi's sarcoma-associated herpesvirus K-bZIP represses gene transcription via SUMO modification', Journal of Virology, 卷 79, 編號 15, 頁 9912-9925. https://doi.org/10.1128/JVI.79.15.9912-9925.2005
Izumiya Y, Ellison TJ, Yeh ETH, Jung JU, Luciw PA, Kung HJ. Kaposi's sarcoma-associated herpesvirus K-bZIP represses gene transcription via SUMO modification. Journal of Virology. 2005 8月 1;79(15):9912-9925. https://doi.org/10.1128/JVI.79.15.9912-9925.2005
Izumiya, Yoshihiro ; Ellison, Thomas J. ; Yeh, Edward T.H. ; Jung, Jae U. ; Luciw, Paul A. ; Kung, Hsing Jien. / Kaposi's sarcoma-associated herpesvirus K-bZIP represses gene transcription via SUMO modification. 於: Journal of Virology. 2005 ; 卷 79, 編號 15. 頁 9912-9925.
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