Kaposi's sarcoma-associated herpesvirus-encoded protein kinase and its interaction with K-bZIP

Yoshihiro Izumiya, Chie Izumiya, Albert Van Geelen, Don Hong Wang, Kit S. Lam, Paul A. Luciw, Hsing Jien Kung

研究成果: 雜誌貢獻文章

51 引文 (Scopus)

摘要

The oncogenic herpesvirus, Kaposi's sarcoma-associated herpesvirus, also identified as human herpesvirus 8, contains genes producing proteins that control transcription and influence cell signaling. Open reading frame 36 (ORF36) of this virus encodes a serine/threonine protein kinase, which is designated the viral protein kinase (vPK). Our recent efforts to elucidate the role of vPK in the viral life cycle have focused on identifying viral protein substrates and determining the effects of vPK-mediated phosphorylation on specific steps in viral replication. The vPK gene was transcribed into 4.2-kb and 3.6-kb mRNAs during the early and late phases of viral reactivation. vPK is colocalized with viral DNA replication/transcription compartments as marked by a polymerase processivity factor, and K-bZIP, a protein known to bind the viral DNA replication origin (Ori-Lyt) and to regulate viral transcription. The vPK physically associated with and strongly phosphorylated K-bZIP at threonine 111, a site also recognized by the cyclin-dependent kinase Cdk2. Both K-bZIP and vPK were corecruited to viral promoters targeted by K-bZIP as well as to the Ori-Lyl region. Phosphorylation of K-bZIP by vPK had a negative impact on K-bZIP transcription repression activity. The extent of posttranslational modification of K-bZIP by sumoylation, a process that influences its repression function, was decreased by vPK phosphorylation at threonine 111. Our data thus identify a new role of vPK as a modulator of viral transcription.
原文英語
頁(從 - 到)1072-1082
頁數11
期刊Journal of Virology
81
發行號3
DOIs
出版狀態已發佈 - 二月 1 2007
對外發佈Yes

指紋

Human herpesvirus 8
Human Herpesvirus 8
viral proteins
Viral Proteins
protein kinases
Protein Kinases
Basic-Leucine Zipper Transcription Factors
transcription (genetics)
replication origin
phosphorylation
Replication Origin
cyclin-dependent kinase
Phosphorylation
Viral DNA
DNA replication
Threonine
DNA Replication
threonine
Sumoylation
Cyclin-Dependent Kinases

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

引用此文

Izumiya, Y., Izumiya, C., Van Geelen, A., Wang, D. H., Lam, K. S., Luciw, P. A., & Kung, H. J. (2007). Kaposi's sarcoma-associated herpesvirus-encoded protein kinase and its interaction with K-bZIP. Journal of Virology, 81(3), 1072-1082. https://doi.org/10.1128/JVI.01473-06

Kaposi's sarcoma-associated herpesvirus-encoded protein kinase and its interaction with K-bZIP. / Izumiya, Yoshihiro; Izumiya, Chie; Van Geelen, Albert; Wang, Don Hong; Lam, Kit S.; Luciw, Paul A.; Kung, Hsing Jien.

於: Journal of Virology, 卷 81, 編號 3, 01.02.2007, p. 1072-1082.

研究成果: 雜誌貢獻文章

Izumiya, Y, Izumiya, C, Van Geelen, A, Wang, DH, Lam, KS, Luciw, PA & Kung, HJ 2007, 'Kaposi's sarcoma-associated herpesvirus-encoded protein kinase and its interaction with K-bZIP', Journal of Virology, 卷 81, 編號 3, 頁 1072-1082. https://doi.org/10.1128/JVI.01473-06
Izumiya Y, Izumiya C, Van Geelen A, Wang DH, Lam KS, Luciw PA 等. Kaposi's sarcoma-associated herpesvirus-encoded protein kinase and its interaction with K-bZIP. Journal of Virology. 2007 2月 1;81(3):1072-1082. https://doi.org/10.1128/JVI.01473-06
Izumiya, Yoshihiro ; Izumiya, Chie ; Van Geelen, Albert ; Wang, Don Hong ; Lam, Kit S. ; Luciw, Paul A. ; Kung, Hsing Jien. / Kaposi's sarcoma-associated herpesvirus-encoded protein kinase and its interaction with K-bZIP. 於: Journal of Virology. 2007 ; 卷 81, 編號 3. 頁 1072-1082.
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