Isosteviol Derivative Inhibits Osteoclast Differentiation and Ameliorates Ovariectomy-Induced Osteoporosis

Huey En Tzeng, Po Hao Huang, Chun Hao Tsai, Gregory J. Tsay, Yi Ju Lee, Tsurng Juhn Huang, Tzu Hung Lin, Ying Ming Chiu, Yi Ying Wu

研究成果: 雜誌貢獻文章

1 引文 斯高帕斯(Scopus)

摘要

NC-8 (ent-16-oxobeyeran-19-N-methylureido) is an isosteviol-derived analogue with multiple biological effects, including anti-inflammation and anti-bacterial activities and inhibition of HBV viral surface antigen gene expression. In this study, we explored the effects of NC-8 on the formation of osteoclasts from RAW 264.7 cells. We found that NC-8 exerts the novel effect of inhibiting osteoclast-like cell formation. Our experiments showed that RANKL-induced ERK, p38, and JNK phosphorylation were inhibited by NC-8. An ovariectomy-induced osteoporosis animal model was used to examine the protective effects of oral treatment with NC-8. Serum analysis was used to examine markers of osteoblasts, osteoclasts, and renal and hepatic function in rats. Micro CT scanning and histological analysis were used to measure bone loss in ovariectomized rats. Oral administration of NC-8 effectively decreased excess bone resorption and significantly antagonized trabecular bone loss in ovariectomized rats. Serum analysis of C-terminal telopeptide of type-I collagen, an osteoclast marker, also showed that NC-8 administration inhibited excess bone resorption. Furthermore, serum analysis showed that renal and liver function were not affected by these doses of NC-8 during long-term treatment. Our results demonstrate that NC-8 inhibits osteoclast differentiation and effectively ameliorates ovariectomy-induced osteoporosis.
原文英語
文章編號11190
期刊Scientific Reports
8
發行號1
DOIs
出版狀態已發佈 - 十二月 1 2018

ASJC Scopus subject areas

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    Tzeng, H. E., Huang, P. H., Tsai, C. H., Tsay, G. J., Lee, Y. J., Huang, T. J., Lin, T. H., Chiu, Y. M., & Wu, Y. Y. (2018). Isosteviol Derivative Inhibits Osteoclast Differentiation and Ameliorates Ovariectomy-Induced Osteoporosis. Scientific Reports, 8(1), [11190]. https://doi.org/10.1038/s41598-018-29257-1