Isoliquiritigenin induces apoptosis and autophagy and inhibits endometrial cancer growth in mice

Chi-Hao Wu, Hsin Yuan Chen, Chia-Woei Wang, Tzong Ming Shieh, Tsui-Chin Huang, Li Chun Lin, Kai Lee Wang, Shih-Min Hsia

研究成果: 雜誌貢獻文章

36 引文 斯高帕斯(Scopus)

摘要

Endometrial cancer is the most common cancer in women, typically with onset after menopause. Isoliquiritigenin (ISL), a licorice flavonoid, was previously shown to have anti-oxidant, anti-inflammatory, and tumor suppression effects. In this study, we investigated the anti-tumor effect of ISL on human endometrial cancer both in vitro and in vivo. We used telomerase-immortalized human endometrial stromal cells (T-HESCs) and human endometrial cancer cell lines (Ishikawa, HEC-1A, and RL95-2 cells) as targets. The effects of ISL on cell proliferation, cell cycle regulation, and apoptosis or autophagy-related protein expression were examined. In addition, we conducted in vivo experiments to confirm the inhibitory effects of ISL on cancer cells. ISL significantly inhibited the viability of cancer cells in a dose- and timedependent manner but with little toxicity on normal cells. In addition, flow cytometry analysis indicated that ISL induced sub-G1 or G2/M phase arrest. ISL treatment activated the extracellular signal regulated kinase signaling pathway to enhance the protein expression of caspase-7/LC3BII associated with apoptosis/autophagy. Furthermore, ISL suppressed xenograft tumor growth in vivo. Taken together, these findings suggest that ISL may induce apoptosis, autophagy, and cell growth inhibition, indicating its potential as a therapeutic agent for human endometrial cancer.
原文英語
頁(從 - 到)73432-73447
頁數16
期刊Oncotarget
7
發行號45
DOIs
出版狀態已發佈 - 2016

ASJC Scopus subject areas

  • Oncology

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