Isolation and cytotoxicity of flavonoids from Daphnis Genkwae Flos

Jer Huei Lin, Ya Tze Lin, Yuh Jan Huang, Kuo Ching Wen, Ruei Ming Chen, Tzuu Huei Ueng, Chun Heng Liao

研究成果: 雜誌貢獻文章同行評審

30 引文 斯高帕斯(Scopus)


For the purpose of quality analysis, we investigated polar constituents as marker substance for some traditional herbs. From Daphnis Genkwae Flos twelve flavonoids were isolated. They were identified as potassium apigenin 7-O-β-D-glucuronate (1), apigenin 7-O-β-D-glucuronide (2), apigenin 7-O-β-D- methylglucuronate (3), apigenin (4), genkwanin 5-O-β-D-primeveroside (5), genkwanin 5-O-β-D-glucoside (6), genkwanin (7), tiliroside (8), kaempferol (9), luteolin 5-O-β-D-glucoside (10), luteolin (11) and 7-O-methylluteolin (12). Among them, 2, 3, 5, 6, 9 and 10 were known compounds, but were for the first time isolated from this material. Compound 1 was isolated from nature for the first time. The structures of 1-12 were established on the basis of their physical properties and spectroscopic evidence. Treatments of human hepatoma HepG2 cells with 0.1 mM apigenin (4), luteolin (11), and 7-O-methylluteolin (12) for 48 hr caused 40% reduction on cell viability, whereas potassium apigenin 7-O-β-D-glucuronate (1), luteolin 5-O-β-D-glucoside (10), genkwanin (7), genkwanin 5-O-β-D-primeveroside (5), and tiliroside (8) caused little or no effects on the viability of HepG2 cell. These data suggest a rough structure - activity relationship of flavonoid cytotoxicity.

頁(從 - 到)6-11
期刊Journal of Food and Drug Analysis
出版狀態已發佈 - 三月 2001

ASJC Scopus subject areas

  • 食品科學
  • 藥理


深入研究「Isolation and cytotoxicity of flavonoids from Daphnis Genkwae Flos」主題。共同形成了獨特的指紋。