Iron and phosphate content of rat ferritin heteropolymers

Shu Hui Juan, Steven D. Aust

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8 引文 斯高帕斯(Scopus)

摘要

An attempt was made to relate the iron and phosphate content of ferritin to its subunit composition. Ferritins from various tissues were separated according to their subunit composition by anion exchange chromatography and according to their iron content by density-gradient centrifugation. Iron and phosphate contents were not related to subunit composition. Recombinant rat liver ferritin heteropolymers of different subunit composition (1, 4, 6, 10, 15, and 17 H chains per 24 mer) were maximally loaded with iron, using ceruloplasmin and phosphate. All loaded approximately the same amount of iron and phosphate (2250 and 380 atoms, respectively). The iron and phosphate content of all ferritin, including the maximally loaded recombinant ferritin heteropolymers, fit an equation we previously reported: [Fe] = 4404 - 5.61 [Pi] (D. deSilva et al., 1993, Arch. Biochem. Biophys. 303, 451-455). These results suggest that the amount of iron and apparently the space within the core of ferritin were not related to different subunit composition.

原文英語
頁(從 - 到)293-298
頁數6
期刊Archives of Biochemistry and Biophysics
357
發行號2
DOIs
出版狀態已發佈 - 九月 15 1998
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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