IONIZING RADIATION-INDUCED CANCER STEMNESS GENE EXPRESSI ON IS REGULATED BY MATRI X NANOTOPOGRAPHY

貢獻的翻譯標題: 奈米表面結構可調控游離輻射所誘導的癌幹細胞基因表現

Long-Sheng Lu, Yin-Ju Chen, Yun-Tien Lin, Jing-Ting Lee, Jeng-Fong Chiou

研究成果: 雜誌貢獻文章

摘要

Introduction: Recent studies have revealed that nanoscale structure (nanotopography) of extracellular matrix is an important regulator of cell biology. However, it remains unknown whether nanotopography regulates cancer cell behaviors upon exposure to ionizing radiation (IR). Materials and Methods: Hepatoma cell lines and a squamous cell carcinoma cell line were cultured on nanopatterned polycaprolactone surfaces and were irradiated. Sphere formation was counted and expression of cancer stemness-related genes (Sox2, Nanog and Oct3/4) was measured with quantitative polymerase chain reaction. Results: In this study, we showed that IR induced expression of stemness genes including Sox2, Nanog and Oct3/4 in several cancer cell lines. Growing J5 hepatoma cells on a square lattice of evenly-spaced nanopits made of polycaprolactone attenuates cancer stemness genes expression induced by IR. Growing J5 cells on a near-square lattice of nanopits has smaller effects on IR-induced cancer stemness genes expression. The phenomenon was reproducible in another cell line derived from squamous cell carcinoma. Conclusions: We provided experimental evidence that radiation-induced expression of cancer stemness genes in cancer cells is regulated by nanotopography of the attaching surface. Further research is warranted to analyze molecular interaction between integrin signaling and cancer stemness, as well as the implications for cancer radiotherapy.
貢獻的翻譯標題奈米表面結構可調控游離輻射所誘導的癌幹細胞基因表現
原文英語
頁(從 - 到)13-20
頁數8
期刊放射治療與腫瘤學
25
發行號1
DOIs
出版狀態已發佈 - 2018

Keywords

  • Nanotopography
  • Extracellular matrix
  • Cancer stemness
  • Ionizing radiation

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