Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

Jam Inn Tzeng, Bing Chang Chen, Huey Mei Chang, Jhi Joung Wang, Munisamy Sureshbabu, Ming Hsien Chien, Ming Jen Hsu, Mauo Ying Bien, Wen Ta Chiu, Chuang Ye Hong, Chien Huang Lin

研究成果: 雜誌貢獻文章

13 引文 (Scopus)

摘要

In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.
原文英語
頁(從 - 到)162-166
頁數5
期刊Pharmacological Research
61
發行號2
DOIs
出版狀態已發佈 - 二月 2010

指紋

Peptidoglycan
Type C Phospholipases
Cyclooxygenase 2
Phosphatidylcholines
Protein Kinase C
Macrophages
Protein C Inhibitor
Protein Kinase Inhibitors
Phosphatidylinositols
Luciferases
tricyclodecane-9-yl-xanthogenate
Ro 31-8220

ASJC Scopus subject areas

  • Pharmacology

引用此文

Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages. / Tzeng, Jam Inn; Chen, Bing Chang; Chang, Huey Mei; Wang, Jhi Joung; Sureshbabu, Munisamy; Chien, Ming Hsien; Hsu, Ming Jen; Bien, Mauo Ying; Chiu, Wen Ta; Hong, Chuang Ye; Lin, Chien Huang.

於: Pharmacological Research, 卷 61, 編號 2, 02.2010, p. 162-166.

研究成果: 雜誌貢獻文章

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abstract = "In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.",
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T1 - Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

AU - Tzeng, Jam Inn

AU - Chen, Bing Chang

AU - Chang, Huey Mei

AU - Wang, Jhi Joung

AU - Sureshbabu, Munisamy

AU - Chien, Ming Hsien

AU - Hsu, Ming Jen

AU - Bien, Mauo Ying

AU - Chiu, Wen Ta

AU - Hong, Chuang Ye

AU - Lin, Chien Huang

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N2 - In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.

AB - In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.

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KW - Protein kinase C

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