Involvement of intracellular calcium mobilization in IL-8 activation in human retinal pigment epithelial cells

I. Hui Yang, Jhen Hong Wong, Che Mai Chang, Ben Kuen Chen, Yao Ting Tsai, Wei Chiao Chen, Eric Terry Wang, Wen Li Hsu, Wei Chiao Chang

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)

摘要

Purpose: Calcium signaling is an important intracellular pathway. Increased intracellular calcium is associated with cytokine regulation and inflammatory signals secretion. The purpose of this study is to understand the molecular mechanisms by which calcium signaling controls IL-8 activation in human RPE cells. Methods: Fluorescence-based calcium imaging and different mutants of IL-8 plasmids were used in this study. The IL-8 promoter activation, gene expression, and secretion were detected by using luciferase reporter assay, quantitative real-time PCR (Q-PCR), and ELISA, respectively. In addition, pharmacological inhibitors and small interfering RNA (siRNA) were applied to clarify the mechanisms of IL-8 activation. Results: Our study reported that intracellular calcium mobilization activated IL-8 gene expression and secretion. Application of pharmacological inhibitor BAY 11-7082, siRNA, and plasmids of the nuclear factor κ light chain enhancer of activated B cells (NF-κB) binding site, we identified that NF-κB is the main transcription factor involved in intracellular calcium mobilization-mediated IL-8 activation in human RPE cells. Conclusions: Collectively, our findings highlight the important role of intracellular calcium mobilization in the activation of IL-8. These findings may be helpful for the clinical applications in the age-related macular degeneration (AMD) prevention and treatment.

原文英語
頁(從 - 到)761-769
頁數9
期刊Investigative Ophthalmology and Visual Science
56
發行號2
DOIs
出版狀態已發佈 - 2015

ASJC Scopus subject areas

  • 眼科
  • 感覺系統
  • 細胞與分子神經科學
  • 醫藥 (全部)

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