Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis

Cheng Kuo Lai, Jing Chi Su, Yu Hsin Lin, Chia Shuo Chang, Chun Lung Feng, Hwai Jeng Lin, Chun Jung Lin, Guan Chin Tseng, Hsin Ho Liu, Jer Tsong Hsieh, Hong Lin Su, Chih Ho Lai

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)

摘要

Aim: The aim of this study was to investigate whether cholesterol plays a pivotal role in cytolethal distending toxin (CDT) mediated pathogenic effects in hosts. Materials & methods: The molecular mechanisms underlying cholesterol sequestering conferred resistance to CDT-induced DNA double-strand breaks (DSBs) and cell cycle arrest were investigated. Histopathological analysis was conducted for evaluating CDT-induced intestinal inflammation in mouse. Results: CDT actions were attenuated by treatment of cells with methyl-β-cyclodextrin (MβCD). Severe intestinal inflammation induced by CDT treatment was observed in high-cholesterol diet-fed mice, but not in normal diet-fed mice, indicating that cholesterol is essential for CDT intoxication. Conclusion: Our findings demonstrate a molecular link between Campylobacter jejuni CDT and cholesterol, which is crucial to facilitate CDT-induced pathogenesis in hosts.

原文英語
頁(從 - 到)489-501
頁數13
期刊Future Microbiology
10
發行號4
DOIs
出版狀態已發佈 - 四月 1 2015

ASJC Scopus subject areas

  • 微生物學
  • 微生物學(醫學)

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