Involvement of β2-microglobulin and integrin α(v)β3 molecules in the coxsackievirus A9 infectious cycle

Martha Triantafilou, Kathy Triantafilou, Keith M. Wilson, Yoshikazu Takada, Nelson Fernandez, Glyn Stanway

研究成果: 雜誌貢獻文章同行評審

40 引文 斯高帕斯(Scopus)

摘要

It is becoming apparent that many viruses employ more than one cell surface molecule for their attachment and cell entry. In this study, we have tested the role of integrin α(v)β3 and MHC class I molecules in the coxsackievirus A9 (CAV-9) infectious cycle. Binding experiments utilizing CHO cells transfected and expressing human integrin α(v)β3, revealed that CAV-9 particles were able to bind to cells, but did not initiate a productive cell infection. Antibodies specific for integrin α(v)β3 molecules significantly reduced CAV-9 infection in susceptible cell lines. Moreover, MAbs specific for β2-microglobulin (β2-m) and MHC class I molecules completely inhibited CAV-9 infection. To assess the effect of these antibodies on virus binding, we analysed CAV-9 binding by flow cytometry in the presence of β2-m- or integrin α(v)β3-specific antibodies. The results showed a reduction in CAV-9 binding in the presence of integrin α(v)β3-specific antibodies while there was no reduction in the presence of β2-m-specific MAb. Taken together, these data suggest that integrin α(v)β3 is required for CAV-9 attachment but is not sufficient for cell entry, while β2-m, although not directly involved in CAV-9 binding, plays a post-attachment role in the CAV-9 infectious process, possibly being involved in virus entry.

原文英語
頁(從 - 到)2591-2600
頁數10
期刊Journal of General Virology
80
發行號10
出版狀態已發佈 - 1999
對外發佈

ASJC Scopus subject areas

  • 免疫學
  • 病毒學

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