Investigation on liquid chromatographic separation of basic compounds using silica column with aqueous/organic mobile phase containing triethylamine and acetic acid

An-Bang Wu, Ming Chun Huang, Hsiu O. Ho, Geng Cheng Yeh, Ming Thau Sheu

研究成果: 雜誌貢獻文章同行評審

9 引文 斯高帕斯(Scopus)

摘要

A high-performance liquid chromatography (HPLC) method using silica column eluted with aqueous solvent mobile phase containing triethylamine (TEA) and acetic acid (ACH) at trace percentages was characterized for the analysis of basic compounds. The key mechanism of this system is ion-exchange accompanying interaction of silanol groups. The increase in the ACH concentration in the mobile phase minimizes the ionization of the silanol group, leading to reduced retention time. However, the greater extent of ionization of silanol caused by the increase of TEA concentration helps to retain basic compounds in the column. Further, the protonated TEA that is positively charged also competes for the ionized silanol group with basic compounds, resulting in the modification of retention time. On the other hand, the retention becomes longer with increasing proportion of either organic or aqueous solvent in mobile phase, and partial replacement of methanol with acetonitrile.

原文英語
頁(從 - 到)443-449
頁數7
期刊Biomedical Chromatography
18
發行號7
DOIs
出版狀態已發佈 - 9月 2004

ASJC Scopus subject areas

  • 分析化學
  • 臨床生物化學
  • 生物化學、遺傳與分子生物學 (全部)
  • 生物化學
  • 藥理

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