Inverted repeats as genetic elements for promoting DNA inverted duplication: Implications in gene amplification

Ching Tai Lin, Wei Hsin Lin, Yi Lisa Lyu, Jacqueline Whang-Peng

研究成果: 雜誌貢獻文章同行評審

35 引文 斯高帕斯(Scopus)


Inverted repeats are important genetic elements for genome instability. In the current study we have investigated the role of inverted repeats in a DNA rearrangement reaction using a linear DNA substrate. We show that linear DNA substrates with terminal inverted repeats can efficiently transform Escherichia coli. The transformation products contain circular inverted dimers in which the DNA sequences between terminal inverted repeats are duplicated. In contrast to the recombination/rearrangement product of circular DNA substrates, which is exclusively one particular form of the inverted dimer, the rearrangement products of the linear DNA substrate consist of two isomeric forms of the inverted dimer. Escherichia coli mutants defective in RecBCD exhibit much reduced transformation efficiency, suggesting a role for RecBCD in the protection rather than destruction of these linear DNA substrates. These results suggest a model in which inverted repeats near the ends of a double-strand break can be processed by a helicase/exonuclease to form hairpin caps. Processing of hairpin capped DNA intermediates can then yield inverted duplications. Linear DNA substrates containing terminal inverted repeats can also be converted into inverted dimers in COS cells, suggesting conservation of this type of genome instability from bacteria to mammalian cells.
頁(從 - 到)3529-3538
期刊Nucleic Acids Research
出版狀態已發佈 - 9月 1 2001

ASJC Scopus subject areas

  • 遺傳學


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