Intrinsically Fluorescent, Stealth Polypyrazoline Nanoparticles with Large Stokes Shift for In Vivo Imaging

Shivshankar R. Mane, I- Lun Hsiao, Masanari Takamiya, Dao Le, Uwe Straehle, Christopher Barner-Kowollik, Carsten Weiss, Guillaume Delaittre

研究成果: 雜誌貢獻文章

8 引文 (Scopus)

摘要

Recent advances in super-resolution microscopy and fluorescence bioimaging allow exploring previously inaccessible biological processes. To this end, there is a need for novel fluorescent probes with specific features in size, photophysical properties, colloidal and optical stabilities, as well as biocompatibility and ability to evade the reticuloendothelial system. Herein, novel fluorescent nanoparticles are introduced based on an inherently fluorescent polypyrazoline (PPy) core and a polyethylene glycol (PEG) shell, which address all aforementioned challenges. Synthesis of the PPy-PEG amphiphilic block copolymer by phototriggered step-growth polymerization is investigated by NMR spectroscopy, size-exclusion chromatography, and mass spectrometry. The corresponding nanoparticles are characterized for their luminescent properties and hydrodynamic size in various aqueous environments (e.g., cell culture media). PPy nanoparticles particularly exhibit a large Stokes shift (Δλ = 160 nm or Δν > 7000 cm −1 ) with visible light excitation and strong colloidal stability. While clearance by macrophages and endothelial cells is minimal, PPy displays good biocompatibility. Finally, PPy nanoparticles prove to be long circulating when injected in zebrafish embryos, as observed by in vivo time-lapse fluorescence microscopy. In summary, PPy nanoparticles are highly promising to be further developed as fluorescent nanodelivery systems with low toxicity and exquisite retention in the blood stream.
原文英語
文章編號1801571
期刊Small
14
發行號36
DOIs
出版狀態已發佈 - 九月 6 2018
對外發佈Yes

指紋

Nanoparticles
Imaging techniques
Biocompatibility
Fluorescence Microscopy
Polyethylene glycols
Biological Phenomena
Mononuclear Phagocyte System
Size exclusion chromatography
Macrophages
Fluorescence microscopy
Endothelial cells
Zebrafish
Hydrodynamics
Fluorescent Dyes
Cell culture
Polymerization
Nuclear magnetic resonance spectroscopy
Block copolymers
Gel Chromatography
Mass spectrometry

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

引用此文

Mane, S. R., Hsiao, I. L., Takamiya, M., Le, D., Straehle, U., Barner-Kowollik, C., ... Delaittre, G. (2018). Intrinsically Fluorescent, Stealth Polypyrazoline Nanoparticles with Large Stokes Shift for In Vivo Imaging. Small, 14(36), [1801571]. https://doi.org/10.1002/smll.201801571

Intrinsically Fluorescent, Stealth Polypyrazoline Nanoparticles with Large Stokes Shift for In Vivo Imaging. / Mane, Shivshankar R.; Hsiao, I- Lun; Takamiya, Masanari; Le, Dao; Straehle, Uwe; Barner-Kowollik, Christopher; Weiss, Carsten; Delaittre, Guillaume.

於: Small, 卷 14, 編號 36, 1801571, 06.09.2018.

研究成果: 雜誌貢獻文章

Mane, SR, Hsiao, IL, Takamiya, M, Le, D, Straehle, U, Barner-Kowollik, C, Weiss, C & Delaittre, G 2018, 'Intrinsically Fluorescent, Stealth Polypyrazoline Nanoparticles with Large Stokes Shift for In Vivo Imaging', Small, 卷 14, 編號 36, 1801571. https://doi.org/10.1002/smll.201801571
Mane, Shivshankar R. ; Hsiao, I- Lun ; Takamiya, Masanari ; Le, Dao ; Straehle, Uwe ; Barner-Kowollik, Christopher ; Weiss, Carsten ; Delaittre, Guillaume. / Intrinsically Fluorescent, Stealth Polypyrazoline Nanoparticles with Large Stokes Shift for In Vivo Imaging. 於: Small. 2018 ; 卷 14, 編號 36.
@article{4d1aeb0773a544a78f5b58f18cb8e196,
title = "Intrinsically Fluorescent, Stealth Polypyrazoline Nanoparticles with Large Stokes Shift for In Vivo Imaging",
abstract = "Recent advances in super-resolution microscopy and fluorescence bioimaging allow exploring previously inaccessible biological processes. To this end, there is a need for novel fluorescent probes with specific features in size, photophysical properties, colloidal and optical stabilities, as well as biocompatibility and ability to evade the reticuloendothelial system. Herein, novel fluorescent nanoparticles are introduced based on an inherently fluorescent polypyrazoline (PPy) core and a polyethylene glycol (PEG) shell, which address all aforementioned challenges. Synthesis of the PPy-PEG amphiphilic block copolymer by phototriggered step-growth polymerization is investigated by NMR spectroscopy, size-exclusion chromatography, and mass spectrometry. The corresponding nanoparticles are characterized for their luminescent properties and hydrodynamic size in various aqueous environments (e.g., cell culture media). PPy nanoparticles particularly exhibit a large Stokes shift (Δλ = 160 nm or Δν > 7000 cm −1 ) with visible light excitation and strong colloidal stability. While clearance by macrophages and endothelial cells is minimal, PPy displays good biocompatibility. Finally, PPy nanoparticles prove to be long circulating when injected in zebrafish embryos, as observed by in vivo time-lapse fluorescence microscopy. In summary, PPy nanoparticles are highly promising to be further developed as fluorescent nanodelivery systems with low toxicity and exquisite retention in the blood stream.",
keywords = "nanomedicine, photopolymerization, tetrazole, visible light, zebrafish",
author = "Mane, {Shivshankar R.} and Hsiao, {I- Lun} and Masanari Takamiya and Dao Le and Uwe Straehle and Christopher Barner-Kowollik and Carsten Weiss and Guillaume Delaittre",
year = "2018",
month = "9",
day = "6",
doi = "10.1002/smll.201801571",
language = "English",
volume = "14",
journal = "Small",
issn = "1613-6810",
publisher = "Wiley-VCH Verlag",
number = "36",

}

TY - JOUR

T1 - Intrinsically Fluorescent, Stealth Polypyrazoline Nanoparticles with Large Stokes Shift for In Vivo Imaging

AU - Mane, Shivshankar R.

AU - Hsiao, I- Lun

AU - Takamiya, Masanari

AU - Le, Dao

AU - Straehle, Uwe

AU - Barner-Kowollik, Christopher

AU - Weiss, Carsten

AU - Delaittre, Guillaume

PY - 2018/9/6

Y1 - 2018/9/6

N2 - Recent advances in super-resolution microscopy and fluorescence bioimaging allow exploring previously inaccessible biological processes. To this end, there is a need for novel fluorescent probes with specific features in size, photophysical properties, colloidal and optical stabilities, as well as biocompatibility and ability to evade the reticuloendothelial system. Herein, novel fluorescent nanoparticles are introduced based on an inherently fluorescent polypyrazoline (PPy) core and a polyethylene glycol (PEG) shell, which address all aforementioned challenges. Synthesis of the PPy-PEG amphiphilic block copolymer by phototriggered step-growth polymerization is investigated by NMR spectroscopy, size-exclusion chromatography, and mass spectrometry. The corresponding nanoparticles are characterized for their luminescent properties and hydrodynamic size in various aqueous environments (e.g., cell culture media). PPy nanoparticles particularly exhibit a large Stokes shift (Δλ = 160 nm or Δν > 7000 cm −1 ) with visible light excitation and strong colloidal stability. While clearance by macrophages and endothelial cells is minimal, PPy displays good biocompatibility. Finally, PPy nanoparticles prove to be long circulating when injected in zebrafish embryos, as observed by in vivo time-lapse fluorescence microscopy. In summary, PPy nanoparticles are highly promising to be further developed as fluorescent nanodelivery systems with low toxicity and exquisite retention in the blood stream.

AB - Recent advances in super-resolution microscopy and fluorescence bioimaging allow exploring previously inaccessible biological processes. To this end, there is a need for novel fluorescent probes with specific features in size, photophysical properties, colloidal and optical stabilities, as well as biocompatibility and ability to evade the reticuloendothelial system. Herein, novel fluorescent nanoparticles are introduced based on an inherently fluorescent polypyrazoline (PPy) core and a polyethylene glycol (PEG) shell, which address all aforementioned challenges. Synthesis of the PPy-PEG amphiphilic block copolymer by phototriggered step-growth polymerization is investigated by NMR spectroscopy, size-exclusion chromatography, and mass spectrometry. The corresponding nanoparticles are characterized for their luminescent properties and hydrodynamic size in various aqueous environments (e.g., cell culture media). PPy nanoparticles particularly exhibit a large Stokes shift (Δλ = 160 nm or Δν > 7000 cm −1 ) with visible light excitation and strong colloidal stability. While clearance by macrophages and endothelial cells is minimal, PPy displays good biocompatibility. Finally, PPy nanoparticles prove to be long circulating when injected in zebrafish embryos, as observed by in vivo time-lapse fluorescence microscopy. In summary, PPy nanoparticles are highly promising to be further developed as fluorescent nanodelivery systems with low toxicity and exquisite retention in the blood stream.

KW - nanomedicine

KW - photopolymerization

KW - tetrazole

KW - visible light

KW - zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85052614121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052614121&partnerID=8YFLogxK

U2 - 10.1002/smll.201801571

DO - 10.1002/smll.201801571

M3 - Article

AN - SCOPUS:85052614121

VL - 14

JO - Small

JF - Small

SN - 1613-6810

IS - 36

M1 - 1801571

ER -