Intranasal gene transfer by chitosan-DNA nanospheres protects BALB/c mice against acute respiratory syncytial virus infection

Mukesh Kumar, Aruna K. Behera, Richard F. Lockey, Jian Zhang, Guraman Bhullar, Cristina Perez de la Cruz, Li Chen Chen, Kam W. Leong, Shau Ku Huang, Shyam S. Mohapatra

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127 引文 斯高帕斯(Scopus)

摘要

Respiratory syncytial virus (RSV) infection is often associated in infancy with life-threatening bronchiolitis, which is also a major risk factor for the development of asthma. At present, no effective prophylaxis is available against RSV infection. Herein, we describe an effective prophylactic intranasal gene transfer strategy utilizing chitosan-DNA nanospheres (IGT), containing a cocktail of plasmid DNAs encoding all RSV antigens, except L. A single administration of IGT (25 μg/mouse) induces expression of the mRNA and proteins of all antigens in the lung and results in a significant reduction of viral titers and viral antigen load after acute RSV infection of these mice. IGT-administered mice show no significant change in airway reactivity to methacholine and no apparent pulmonary inflammation. Furthermore, IGT results in significant induction of RSV-specific IgG antibodies, nasal IgA antibodies, cytotoxic T lymphocytes, and interferon-γ production in the lung and splenocytes compared with controls. Together, these results demonstrate the potential of IGT against acute RSV infection.

原文英語
頁(從 - 到)1415-1425
頁數11
期刊Human Gene Therapy
13
發行號12
DOIs
出版狀態已發佈 - 八月 10 2002
對外發佈

ASJC Scopus subject areas

  • 分子醫學
  • 分子生物學
  • 遺傳學

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