Intra-articular injection of the selective cyclooxygenase-2 inhibitor meloxicam (Mobic) reduces experimental osteoarthritis and nociception in rats

Z. H. Wen, C. C. Tang, Y. C. Chang, S. Y. Huang, C. H. Chen, S. C. Wu, S. P. Hsieh, C. S. Hsieh, K. Y. Wang, S. Y. Lin, H. L. Lee, C. H. Lee, H. C. Kuo, W. F. Chen, Y. H. Jean

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15 引文 斯高帕斯(Scopus)

摘要

Objective: To study the effect of intra-articular injection of meloxicam (Mobic) on the development of osteoarthritis (OA) in rats and examine concomitant changes in nociceptive behavior and the expression of mitogen-activated protein kinases (MAPKs) in articular cartilage chondrocytes. Methods: OA was induced in Wistar rats by right anterior cruciate ligament transection (ACLT); the left knee was not treated. The OA+meloxicam (1.0mg) group was injected intra-articularly in the ACLT knee with 1.0mg of meloxicam once a week for 5 consecutive weeks starting 5 weeks after ACLT. The OA+meloxicam (0.25mg) group was treated similarly with 0.25mg meloxicam. The sham group underwent arthrotomy only and received vehicle of 0.1mL sterile 0.9% saline injections, whereas the naive rats in meloxicam-only groups were treated similarly with 1.0- and 0.25-mg meloxicam. Nociception was measured as secondary mechanical allodynia and hind paw weight-bearing distribution at before (pre-) and 5, 10, 15, and 20 weeks post-ACLT. Histopathology of the cartilage and synovia was examined 20 weeks after ACLT. Immunohistochemical analysis was performed to examine the effect of meloxicam on MAPKs (p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK)) expression in the articular cartilage chondrocytes. Results: OA rats receiving intra-articular meloxicam treatment showed significantly less cartilage degeneration and synovitis than saline-treated controls. Nociception were improved in the OA+meloxicam groups compared with the OA group. Moreover, meloxicam attenuated p38 and JNK but enhanced ERK expression in OA-affected cartilage. Conclusions: Intra-articular injection of meloxicam (1) attenuates the development of OA, (2) concomitantly reduces nociception, and (3) modulates chondrocyte metabolism, possibly through inhibition of cellular p38 and JNK, but enhances ERK expression.

原文英語
頁(從 - 到)1976-1986
頁數11
期刊Osteoarthritis and Cartilage
21
發行號12
DOIs
出版狀態已發佈 - 十二月 2013

ASJC Scopus subject areas

  • 生物醫學工程
  • 骨科和運動醫學
  • 風濕病

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