Intra- and intermolecular strand transfer by HeLa DNA topoisomerase I.

B. D. Halligan, J. L. Davis, K. A. Edwards, Leroy-Fong Liu

研究成果: 雜誌貢獻文章

85 引文 (Scopus)

摘要

The major type I DNA topoisomerase (topo I) has been purified from HeLa cell nuclei to a homogeneous, monomeric protein (Mr = 100,000). Similar to the nicking-closing enzyme (Mr = 67,000) from rat liver (Been, M. D., and Champoux, J. J. (1981) Proc. Natl. Acad. Sci. U. S. A. 78, 2883-2887), HeLa topo I has the following properties: (a) HeLa topo I breaks down single-stranded DNA to smaller fragments, each with an enzyme-linked 3'-phosphoryl end and a free 5'-OH end. This cleavage is not dependent upon protein denaturant or protease treatment. (b) HeLa topo I produces single-stranded DNA circles from linear single-stranded DNA. Such DNA circles are believed to be produced by the intramolecular cyclization of topo I-linked, single-stranded DNA fragments. (c) HeLa topo I-linked, single-stranded fragments (donors) can join covalently to double-stranded DNA possessing a 5'-OH group (acceptors). The donor is transferred to the 5'-OH end of the acceptor, independent of the position of the end (internal nick or end of linear DNA) or the configuration of the end (flush, 5'-protruding, or 5'-recessed end) of the acceptor. (d) HeLa topo I cleavage of single-stranded DNA is site-specific, but no special sequence at the ends of the acceptor molecule is apparently required for a successful heterologous strand transfer. These results suggest that HeLa topo I may be involved in DNA sequence rearrangements in addition to its possible role as a swivelase for transcription and replication.

原文英語
頁(從 - 到)3995-4000
頁數6
期刊Journal of Biological Chemistry
257
發行號7
出版狀態已發佈 - 四月 10 1982
對外發佈Yes

指紋

Type I DNA Topoisomerase
Single-Stranded DNA
DNA
Single-Stranded DNA Breaks
Gene Rearrangement
Enzymes
Cyclization
Cell Nucleus
HeLa Cells
DNA sequences
Transcription
Proteins
Peptide Hydrolases
Liver
Rats
Cells
Molecules

ASJC Scopus subject areas

  • Biochemistry

引用此文

Halligan, B. D., Davis, J. L., Edwards, K. A., & Liu, L-F. (1982). Intra- and intermolecular strand transfer by HeLa DNA topoisomerase I. Journal of Biological Chemistry, 257(7), 3995-4000.

Intra- and intermolecular strand transfer by HeLa DNA topoisomerase I. / Halligan, B. D.; Davis, J. L.; Edwards, K. A.; Liu, Leroy-Fong.

於: Journal of Biological Chemistry, 卷 257, 編號 7, 10.04.1982, p. 3995-4000.

研究成果: 雜誌貢獻文章

Halligan, BD, Davis, JL, Edwards, KA & Liu, L-F 1982, 'Intra- and intermolecular strand transfer by HeLa DNA topoisomerase I.', Journal of Biological Chemistry, 卷 257, 編號 7, 頁 3995-4000.
Halligan, B. D. ; Davis, J. L. ; Edwards, K. A. ; Liu, Leroy-Fong. / Intra- and intermolecular strand transfer by HeLa DNA topoisomerase I. 於: Journal of Biological Chemistry. 1982 ; 卷 257, 編號 7. 頁 3995-4000.
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