Interlocking of plasmid DNAs due to lac repressor-operator interaction

Hai Young Wu, Kawai Lau, Leroy F. Liu

研究成果: 雜誌貢獻文章同行評審

4 引文 斯高帕斯(Scopus)

摘要

The presence of a single lac repressor binding sequence on plasmid DNAs is shown to mediate the formation of interlocked dimers in E. coli. The presence of both homo- and hetero-interlocked dimers suggests that the lac repressor complex can bring together randomly two plasmid DNA molecules to facilitate gyrase-mediated interlocking. The exclusive formation of multiply intertwined dimers also suggests that the lac repressor complex may bind simultaneously to a pair of replicated daughter plasmid molecules prior to their segregation. The formation of interlocked plasmid DNAs can be indicative of interaction between two DNA bound proteins in vivo.

原文英語
頁(從 - 到)1104-1114
頁數11
期刊Journal of Molecular Biology
228
發行號4
DOIs
出版狀態已發佈 - 十二月 20 1992

ASJC Scopus subject areas

  • 結構生物學
  • 分子生物學

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