Interference of selected clinical medicines on DRI® and TDx® immunoassays of morphine and methamphetamine in urine

S. G. Chang, C. S. Chien, H. M. Lee, C. Y. Chen

研究成果: 雜誌貢獻文章

3 引文 (Scopus)

摘要

Eleven commonly used antihistamines, nonsteroidal anti-inflammatory drugs, and vitamins were evaluated in vitro for potential interference with DRI® and TDx® immunoassay reagents for opiates and amphetamines. Mechanisms for the observed interference were also explored. DRI® reagents appear to be more susceptible to interference by the compounds studied. Regarding mechanistic aspects, tolmetin was found to be strongly absorbent at the detection wavelength (340 nm) causing a false negative response; diphenhydramine, pheniramine, and trimethobenzamide appeared to cause false positive interference through their affinity to the antibodies used in the DRI® reagents; while chlorpromazine was found to positively interfere with the assay through both cross reaction and UV absorption. Ascorbic acid, when in high concentrations (>2%, w/v), may inhibit the enzyme (G6PDH) activity and result in a false negative response.
原文英語
頁(從 - 到)259-268
頁數10
期刊Journal of Food and Drug Analysis
7
發行號4
出版狀態已發佈 - 1999

指紋

Methamphetamine
morphine
Clinical Medicine
crossover interference
immunoassays
Immunoassay
Morphine
medicine
urine
Urine
Opiate Alkaloids
Pheniramine
Tolmetin
Amphetamines
Diphenhydramine
amphetamines
Antibody Affinity
Histamine Antagonists
Cross Reactions
Chlorpromazine

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

引用此文

Interference of selected clinical medicines on DRI® and TDx® immunoassays of morphine and methamphetamine in urine. / Chang, S. G.; Chien, C. S.; Lee, H. M.; Chen, C. Y.

於: Journal of Food and Drug Analysis, 卷 7, 編號 4, 1999, p. 259-268.

研究成果: 雜誌貢獻文章

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