Interaction of WW domains with hematopoietic transcription factor p45/NF-E2 and RNA polymerase II

Narender R. Gavva, Rama Gavva, Kira Ermekova, Marius Sudol, C. K.James Shen

研究成果: 雜誌貢獻文章同行評審

60 引文 斯高帕斯(Scopus)

摘要

NF-E2 is an erythroid-specific transcription factor required for expression of several erythroid-specific genes. By Far-Western blotting and yeast two-hybrid assay, we demonstrate that p45, the large subunit of NF-E2, is capable of binding to a specific set of WW domain-containing proteins, including the ubiquitin ligase hRPF1. This binding is mediated through the interaction between the WW domains and a PY motif located within the amino- terminal region of p45. Interestingly, the carboxyl-terminal domain of mammalian RNA polymerase II binds a similar set of WW domains to which p45 interacts with. We discuss the data in terms of possible new pathways through which the processes of transcriptional regulation by NF-E2 could be regulated in erythroid and megakaryote cells.

原文英語
頁(從 - 到)24105-24108
頁數4
期刊Journal of Biological Chemistry
272
發行號39
DOIs
出版狀態已發佈 - 9月 26 1997
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 分子生物學
  • 細胞生物學

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