Interaction between the left-handed Z-DNA and polyamine-2: The crystal structure of the d(CG)3 and spermidine complex

Hirofumi Ohishi, Isao Nakanishi, Kouichi Inubushi, Gijs Van Der Marel, Jacques H. Van Boom, Alexander Rich, Andrew H.J. Wang, Toshio Hakoshima, Ken Ichi Tomita

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22 引文 斯高帕斯(Scopus)

摘要

This paper deals with the crystal structure of d(CG)3-spermidine complex. The DNA fragment, d(CG)3, was crystallized with N-(2-amino-propyl)- 1,4-diamino-butane, PA(34), spermidine. The results of its X-ray crystallographic analysis showed many intermolecular contacts between d(CG)3 and spermidine, but the binding mode of spermidine to the d(CG)3 molecule is different from that of the d(CG)3 and N-(2-amino-ethyl)-1,4-diamino-butane [PA(24)] complex: a spermidine molecule bound to the d(CG)3 and its symmetrically related neighboring d(CG)3 molecules through the water molecules with hydrogen bonds, while one PA(24) molecule connected directly to one d(CG)3 molecule, but not to its neighboring d(CG)3 molecule. In the crystal, the d(CG)3 molecule was the left-handed Z-form, and three magnesium cations and a sodium cation were observed around the d(CG)3 moiety with different binding modes from the case of the d(CG)3-PA(24) complex.

原文英語
頁(從 - 到)153-156
頁數4
期刊FEBS Letters
391
發行號1-2
DOIs
出版狀態已發佈 - 8月 5 1996
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 結構生物學
  • 生物化學
  • 分子生物學
  • 遺傳學
  • 細胞生物學

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