The 5' end of avian sarcoma and leukosis virus RNA near the primer binding site forms two RNA secondary structures, U5-inverted repeat (U5-IR) and U5- leader stems, which are required for efficient initiation of reverse transcription. Lying between these two secondary structures is a 7-base sequence that can anneal to the TψC loop of the tRNA(Trp) primer. Base substitutions in U5 RNA which disrupt this potential interaction result in a defect in the initiation of reverse transcription both in vivo and in vitro. The defect can be complemented in vitro by base substitutions in the primer. The U5 RNA-TψC interaction is also dependent upon the presence of both the U5-IR and the U5-leader structures. These RNA secondary structures and primer interactions are conserved in other type C and D retroviruses, suggesting that there is a common mechanism for the initiation of reverse transcription in all of these retroviruses.
|頁（從 - 到）||2464-2472|
|期刊||Journal of Virology|
|出版狀態||已發佈 - 一月 1 1992|
ASJC Scopus subject areas
Aiyar, A., Cobrinik, D., Ge, Z., Kung, H. J., & Leis, J. (1992). Interaction between retroviral U5 RNA and the TψC loop of the tRNA(Trp) primer is required for efficient initiation of reverse transcription. Journal of Virology, 66(4), 2464-2472.