Interaction between retroviral U5 RNA and the TψC loop of the tRNA(Trp) primer is required for efficient initiation of reverse transcription

A. Aiyar, D. Cobrinik, Z. Ge, H. J. Kung, J. Leis

研究成果: 雜誌貢獻文章

120 引文 斯高帕斯(Scopus)

摘要

The 5' end of avian sarcoma and leukosis virus RNA near the primer binding site forms two RNA secondary structures, U5-inverted repeat (U5-IR) and U5- leader stems, which are required for efficient initiation of reverse transcription. Lying between these two secondary structures is a 7-base sequence that can anneal to the TψC loop of the tRNA(Trp) primer. Base substitutions in U5 RNA which disrupt this potential interaction result in a defect in the initiation of reverse transcription both in vivo and in vitro. The defect can be complemented in vitro by base substitutions in the primer. The U5 RNA-TψC interaction is also dependent upon the presence of both the U5-IR and the U5-leader structures. These RNA secondary structures and primer interactions are conserved in other type C and D retroviruses, suggesting that there is a common mechanism for the initiation of reverse transcription in all of these retroviruses.
原文英語
頁(從 - 到)2464-2472
頁數9
期刊Journal of Virology
66
發行號4
出版狀態已發佈 - 一月 1 1992
對外發佈Yes

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)
  • Virology

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