Interaction between left-handed Z-DNA and polyamine 3. The crystal structure of the d(CG)3 and thermospermine complex

Hirofumi Ohishi, Noriko Terasoma, Isao Nakanishi, Gijs Van Der Marel, Jacques H. Van Boom, Alexander Rich, Andrew H.J. Wang, Toshio Hakoshima, Ken Ichi Tomita

研究成果: 雜誌貢獻文章同行評審

22 引文 斯高帕斯(Scopus)

摘要

The DNA fragment, d(CG)3, was co-crystallized with N-(3-amino-propyl)-N-(5-aminopropyl)-1,4-diaminobutane (thermospermine; PA(334)), a polyamine metabolized from the nucleic acid. By using a good crystal with dimensions of 0.5 x 0.5 x 0.5 mm3, X-ray intensity data were collected up to 1.0 Å resolution. Two thermospermine molecules and a magnesium cation mere bound to the left-handed double-helical d(CG)3 molecule. The d(CC)3 molecule adopted the left-handed Z-conformation and two thermospermine molecules and a magnesium cation neutralized the negative charges of the phosphate groups of the d(CG)3 molecule. Furthermore, the binding modes between d(CG)3 and thermospermine were different from those of d(CG)3 complexes with PA(24), spermidine and spermine. This is the first case in which it was determined by X-ray crystallographic analysis that one of two thermospermine molecules bound three d(CG)3 duplexes which were symmetrically related to each other, and the other formed two hydrogen bonds at the N(5) and N(9) atoms with two adjacent nucleotide phosphate groups of a single d(CG)3 strand at the minor groove. Furthermore, no direct coordination bond was found between the d(CG)3 molecule and the magnesium cation.

原文英語
頁(從 - 到)291-296
頁數6
期刊FEBS Letters
398
發行號2-3
DOIs
出版狀態已發佈 - 12月 2 1996
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 結構生物學
  • 生物化學
  • 分子生物學
  • 遺傳學
  • 細胞生物學

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