Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment

Li-Chern Pan, Fang Chi Hsu, Yun Ying Wu, Fan Gang Tseng, Da Jen Yao, Yieh Loong Tsai, Jiann-Loung Hwang

研究成果: 書貢獻/報告類型會議貢獻

摘要

Since male abnormality has now been reported to account at least thirty percent of couples with fertility problem. Therefore, distinguishing and classification of sperm has become more and more important, especially when Assisted Reproductive Technology (ART) like in vitro fertilization (IVF) is involved. However, traditional sperm sorting methods all requires manual selection, which is tedious and often cause sperm damage during the screen process. Although, new design from our recent study, seemed to point for a possibility to make these kind of MISS device capable of separating sperms into various mobility sub-class. In addition, the Hoechst 33258 and propidium iodide double staining results, also confirmed that the most motile and health sperm is indeed able to leave the laminar stream and being sorted out at outlet-h, with least dead sperm or debris. Meanwhile, we observed the dead sperm had been dominate from clear red stains and live sperm are not as active as is corresponded with more diffused blue stains. In order to confirm the real sorting situation, we counted the amount of sperm in collected sample by flow cytometry and tried to establish the statistic model. Though the flow cytometry, we could detect the sperm fluorescent distribution and count the size of sperm population. Nevertheless, t-test with 95% confidence level also shown significant difference in live/dead sperm ratio. Therefore, from the combined result among flow cytometry, fluorescent stain, and the statistic model test, it is confirmed that live sperms were able to cross the laminar stream created by the device and reach the sorting chamber with corresponding range from non-motile sperms to debris, as well as from living to dead ones. It is concluded that the proposed method is cost effective and is possible to serve as a protocol for rapid sperm quantitative assessment.
原文英語
主出版物標題IFMBE Proceedings
頁面332-335
頁數4
25
版本8
DOIs
出版狀態已發佈 - 2009
事件World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors - Munich, 德国
持續時間: 九月 7 2009九月 12 2009

其他

其他World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors
國家德国
城市Munich
期間9/7/099/12/09

指紋

Flow cytometry
Fluidics
Sorting
Coloring Agents
Debris
Statistics
Bisbenzimidazole
Propidium
Health
Costs

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

引用此文

Pan, L-C., Hsu, F. C., Wu, Y. Y., Tseng, F. G., Yao, D. J., Tsai, Y. L., & Hwang, J-L. (2009). Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment. 於 IFMBE Proceedings (8 編輯, 卷 25, 頁 332-335) https://doi.org/10.1007/978-3-642-03887-7-96

Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment. / Pan, Li-Chern; Hsu, Fang Chi; Wu, Yun Ying; Tseng, Fan Gang; Yao, Da Jen; Tsai, Yieh Loong; Hwang, Jiann-Loung.

IFMBE Proceedings. 卷 25 8. 編輯 2009. p. 332-335.

研究成果: 書貢獻/報告類型會議貢獻

Pan, L-C, Hsu, FC, Wu, YY, Tseng, FG, Yao, DJ, Tsai, YL & Hwang, J-L 2009, Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment. 於 IFMBE Proceedings. 8 edn, 卷 25, 頁 332-335, World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors, Munich, 德国, 9/7/09. https://doi.org/10.1007/978-3-642-03887-7-96
Pan, Li-Chern ; Hsu, Fang Chi ; Wu, Yun Ying ; Tseng, Fan Gang ; Yao, Da Jen ; Tsai, Yieh Loong ; Hwang, Jiann-Loung. / Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment. IFMBE Proceedings. 卷 25 8. 編輯 2009. 頁 332-335
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