Intérêt d'une combinaison gélatine-agarose cryodesséchée pour l'extraction sélective de la fibronectine à partir du plasma humain

T. Burnouf, M. Allary, J. Guilbaud, E. Boschetti, J. Saint-Blancard

研究成果: 雜誌貢獻文章

2 引文 斯高帕斯(Scopus)

摘要

We report here a simple procedure for the isolation of human plasma fibronectin by affinity adsorption on a new lyophylized adsorbent which is obtained by mixing and polymerizing agarose with gelatin. This method permits to obtain fibronectin and a fibronectin-depleted plasma without dilution. The yield of fibronectin is about 20 to 25 per cent. Fibronectin was identified by cross-reactions against specific antibodies. Its purity (≥99 per cent) was controled by immunoelectrophoresis and SDS-PAGE. Furthermore, its biological activity was demonstrated by a spreading test performed on BHK cells. Such a test can be used to determine the specific activity of extracted fibronectin considering that the number of spreaded cells placed in presence of exogenous fibronectin (5 μg/ml of culture medium) was 4.5 to 5 fold-superior to the control, after 30 minutes of incubation at 37°C.
原文法語
頁(從 - 到)559-570
頁數12
期刊Revue Francaise de Transfusion et Immuno-Hematologie
24
發行號6
DOIs
出版狀態已發佈 - 1981
對外發佈Yes

ASJC Scopus subject areas

  • Medicine(all)
  • Hematology

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