TY - JOUR
T1 - Inhibitory effects of glycosides from the leaves of Melaleuca quinquenervia on vascular contraction of rats
AU - Lee, Tzong Huei
AU - Wang, Guei Jane
AU - Lee, Ching Kuo
AU - Kuo, Yueh Hsiung
AU - Chou, Chang Hung
PY - 2002
Y1 - 2002
N2 - Two new glycosides, 3-hydroxy-5-methoxy-4-methylphenyl β-D-glucopyranoside (1) and 4-benzoyl-2-C-β-glucopyranosyl-3,5-dihydroxy-6-methylphenyl β-D-glucopyranoside (2), together with four known glycosides, 2-endo-β-o-glucopyranosyloxy-1,8-cineole (3a), 2-exo-β-D-glucopyranosyloxy-1,8-cineole (3b), roseoside (4), and citroside A (5), were isolated from the methanolic extract of leaves of Melaleuca quinquenervia. Their structures were elucidated on the basis of spectroscopic analysis. Compounds 1, 2a and 3 inhibited contractile response induced by phenylephrine in aortic rings from Sprague-Dawley rats. This inhibition was independent of the endothelium. Compounds 2 and 4 significantly relaxed precontracted aortic rings, in an endothelium-dependent manner. Pretreatment of Nω-nitro-L-arginine (L-NNA), a nitric oxide synthase inhibitor, partially attenuated the vasorelaxation induced by both compounds, suggesting that nitric oxide was likely the responsible mediator. The rank-order potency (EC50 value) of vasorelaxing activities of these compounds is 4 > 2 > 2a > 3 > 1.
AB - Two new glycosides, 3-hydroxy-5-methoxy-4-methylphenyl β-D-glucopyranoside (1) and 4-benzoyl-2-C-β-glucopyranosyl-3,5-dihydroxy-6-methylphenyl β-D-glucopyranoside (2), together with four known glycosides, 2-endo-β-o-glucopyranosyloxy-1,8-cineole (3a), 2-exo-β-D-glucopyranosyloxy-1,8-cineole (3b), roseoside (4), and citroside A (5), were isolated from the methanolic extract of leaves of Melaleuca quinquenervia. Their structures were elucidated on the basis of spectroscopic analysis. Compounds 1, 2a and 3 inhibited contractile response induced by phenylephrine in aortic rings from Sprague-Dawley rats. This inhibition was independent of the endothelium. Compounds 2 and 4 significantly relaxed precontracted aortic rings, in an endothelium-dependent manner. Pretreatment of Nω-nitro-L-arginine (L-NNA), a nitric oxide synthase inhibitor, partially attenuated the vasorelaxation induced by both compounds, suggesting that nitric oxide was likely the responsible mediator. The rank-order potency (EC50 value) of vasorelaxing activities of these compounds is 4 > 2 > 2a > 3 > 1.
KW - Glycosides
KW - Leaves
KW - Melaleuca quinquenervia
KW - Myrtaceae
KW - Vaso-relaxing activities
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U2 - 10.1055/s-2002-32563
DO - 10.1055/s-2002-32563
M3 - Article
C2 - 12094289
AN - SCOPUS:0036070058
VL - 68
SP - 492
EP - 496
JO - Planta Medica
JF - Planta Medica
SN - 0032-0943
IS - 6
ER -