Inhibitory effects of a rice hull constituent on tumor necrosis factor α, prostaglandin E2, and cyclooxygenase-2 production in lipopolysaccharide-activated mouse macrophages

Sheng Tung Huang, Chien-Tsu Chen, Kur Ta Chieng, Shih Hao Huang, Been Huang Chiang, Leng-Fang Wang, Hsien Saw Kuo, Chun-Mao Lin

研究成果: 雜誌貢獻文章同行評審

38 引文 斯高帕斯(Scopus)

摘要

Isovitexin, isolated from rice hull of Oryza sativa, has been characterized as a potent antioxidant. Its antioxidant activity, determined on the basis of inhibition of lipid peroxidation by the Fenton reaction, was comparable with that of α-tocopherol, a well-established antioxidant. Isovitexin was able to reduce the amount of hydrogen peroxide production induced by lipopolysaccharide (LPS) in mouse macrophage RAW264.7 cells. In this study, we assessed its effects on the production of tumor necrosis factor α(TNF-α), prostaglandin E2 (PGE2), and the expression of cyclooxygenase-2 (COX-2) in LPS-activated RAW 264.7 macrophages. Isovitexin inhibited the release of TNF-α, a proinflammatory cytokine, upon LPS activation with a 50% inhibitory concentration (IC50) of 78.6 μM. Isovitexin markedly reduced LPS-stimulated PGE2 production in a concentration-dependent manner, with an IC50 of 80.0 μM. The expression of COX-2 was also inhibited by isovitexin treatment. Our results suggest that suppression of ROS-mediated COX-2 expression by isovitexin is beneficial in reducing inflammation and carcinogenesis.
原文英語
頁(從 - 到)387-395
頁數9
期刊Annals of the New York Academy of Sciences
1042
DOIs
出版狀態已發佈 - 2005

ASJC Scopus subject areas

  • 生物化學、遺傳與分子生物學 (全部)
  • 科學史與哲學

指紋

深入研究「Inhibitory effects of a rice hull constituent on tumor necrosis factor α, prostaglandin E2, and cyclooxygenase-2 production in lipopolysaccharide-activated mouse macrophages」主題。共同形成了獨特的指紋。

引用此