Inhibitory effect of stevioside on calcium influx to produce antihypertension

Chun-Nin Lee, K. L. Wong, J. C. Liu, Y. J. Chen, J. T. Cheng, P. Chan

研究成果: 雜誌貢獻文章

111 引文 (Scopus)

摘要

Stevioside is a sweet-tasting glycoside occurring abundantly in the leaves of Stevia rebaudiana (Compositae). It has been used popularly in Japan and Brazil as a sugar substitute for decades. Previous study has shown that it lowered blood pressure in spontaneously hypertensive rats (SHRs) when administered intravenously. This study shows that intraperitoneal injection of stevioside 25 mg/kg also has antihypertensive effect in SHRs. In isolated aortic rings from normal rats, stevioside could dose-dependently relax the vasopressin-induced vasoconstriction in both the presense and absence of endothelium. However, stevioside had no effect on phenylephrine- and KCI-induced phasic vasoconstriction. In addition, stevioside lost its influence on vasopressin-induced vasoconstriction in Ca2+-free medium. The results indicate that stevioside caused vasorelaxation via an inhibition of Ca2+ influx into the blood vessel. This phenomenon was further confirmed in cultured aortic smooth muscle cells (A7r5). Using 10-5M methylene blue for 15 min, stevioside could still relax 10-8 M vasopressin-induced vasoconstriction in isolated rat aortic rings, showing that this vasorelaxation effect was not related to nitric oxide. The present data show that the vasorelexation effect of stevioside was mediated mainly through Ca2+ influx inhibition.
原文英語
頁(從 - 到)796-799
頁數4
期刊Planta Medica
67
發行號9
DOIs
出版狀態已發佈 - 2001

指紋

stevioside
Calcium
calcium
vasoconstriction
Vasoconstriction
vasopressin
Rats
Vasopressins
vasodilation
rats
Inbred SHR Rats
Vasodilation
Stevia
sugar substitutes
Stevia rebaudiana
Sweetening Agents
antihypertensive effect
phenylephrine
Asteraceae
methylene blue

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

引用此文

Inhibitory effect of stevioside on calcium influx to produce antihypertension. / Lee, Chun-Nin; Wong, K. L.; Liu, J. C.; Chen, Y. J.; Cheng, J. T.; Chan, P.

於: Planta Medica, 卷 67, 編號 9, 2001, p. 796-799.

研究成果: 雜誌貢獻文章

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abstract = "Stevioside is a sweet-tasting glycoside occurring abundantly in the leaves of Stevia rebaudiana (Compositae). It has been used popularly in Japan and Brazil as a sugar substitute for decades. Previous study has shown that it lowered blood pressure in spontaneously hypertensive rats (SHRs) when administered intravenously. This study shows that intraperitoneal injection of stevioside 25 mg/kg also has antihypertensive effect in SHRs. In isolated aortic rings from normal rats, stevioside could dose-dependently relax the vasopressin-induced vasoconstriction in both the presense and absence of endothelium. However, stevioside had no effect on phenylephrine- and KCI-induced phasic vasoconstriction. In addition, stevioside lost its influence on vasopressin-induced vasoconstriction in Ca2+-free medium. The results indicate that stevioside caused vasorelaxation via an inhibition of Ca2+ influx into the blood vessel. This phenomenon was further confirmed in cultured aortic smooth muscle cells (A7r5). Using 10-5M methylene blue for 15 min, stevioside could still relax 10-8 M vasopressin-induced vasoconstriction in isolated rat aortic rings, showing that this vasorelaxation effect was not related to nitric oxide. The present data show that the vasorelexation effect of stevioside was mediated mainly through Ca2+ influx inhibition.",
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AU - Liu, J. C.

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AU - Cheng, J. T.

AU - Chan, P.

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