Inhibitory effect of Anoectochilus formosanus extract on hyperglycemia-related PD-L1 expression and cancer proliferation

Yih Ho, Yan Fang Chen, Li Hsuan Wang, Kuang Yang Hsu, Yu Tang Chin, Yu Chen S.H. Yang, Shwu Huey Wang, Yi Ru Chen, Ya Jung Shih, Leroy F. Liu, Kuan Wang, Jacqueline Whang-Peng, Heng Yuan Tang, Hung Yun Lin, Hsuan Liang Liu, Shwu Jiuan Lin

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

Traditional herb medicine, golden thread (Anoectochilus formosanus Hayata) has been used to treat various diseases. Hyperglycemia induces generation of reactive oxygen species (ROS) and enhancement of oxidative stress which are risk factors for cancer progression and metastasis. In this study, we evaluated hypoglycemic effect of A. formosanus extracts (AFEs) in an inducible hyperglycemia animal model and its capacity of free-radical scavenging to establish hyperglycemia-related carcinogenesis. AFE reduced blood glucose in hyperglycemic mice while there was no change in control group. The incremental area under blood glucose response curve was decreased significantly in hyperglycemic mice treated with AFE in a dose-dependent manner. AFE and metformin at the same administrated dose of 50 mg/kg showed similar effect on intraperitoneal glucose tolerance test in hyperglycemic mice. Free-radical scavenger capacity of AFE was concentration dependent and 200 μg/ml of AFE was able to reduce more than 41% of the free radical. Treatment of cancer cells with AFE inhibited constitutive PD-L1 expression and its protein accumulation. It also induced expression of pro-apoptotic genes but inhibited proliferative and metastatic genes. In addition, it induced anti-proliferation in cancer cells. The results suggested that AFE not only reduced blood glucose concentration as metformin but also showed its potential use in cancer immune chemoprevention/therapy via hypoglycemic effect, ROS scavenging and PD-L1 suppression.
原文英語
文章編號807
期刊Frontiers in Pharmacology
9
發行號AUG
DOIs
出版狀態已發佈 - 八月 2 2018

指紋

Obese Mice
Hyperglycemia
Blood Glucose
Metformin
Hypoglycemic Agents
Free Radicals
Reactive Oxygen Species
Neoplasms
Free Radical Scavengers
Chemoprevention
Traditional Medicine
Glucose Tolerance Test
Genes
Carcinogenesis
Oxidative Stress
Animal Models
Neoplasm Metastasis
Control Groups
Therapeutics
Proteins

Keywords

  • Anti-proliferation
  • Cancer
  • Hyperglycemia
  • Metformin
  • PD-L1

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

引用此文

Inhibitory effect of Anoectochilus formosanus extract on hyperglycemia-related PD-L1 expression and cancer proliferation. / Ho, Yih; Chen, Yan Fang; Wang, Li Hsuan; Hsu, Kuang Yang; Chin, Yu Tang; Yang, Yu Chen S.H.; Wang, Shwu Huey; Chen, Yi Ru; Shih, Ya Jung; Liu, Leroy F.; Wang, Kuan; Whang-Peng, Jacqueline; Tang, Heng Yuan; Lin, Hung Yun; Liu, Hsuan Liang; Lin, Shwu Jiuan.

於: Frontiers in Pharmacology, 卷 9, 編號 AUG, 807, 02.08.2018.

研究成果: 雜誌貢獻文章

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abstract = "Traditional herb medicine, golden thread (Anoectochilus formosanus Hayata) has been used to treat various diseases. Hyperglycemia induces generation of reactive oxygen species (ROS) and enhancement of oxidative stress which are risk factors for cancer progression and metastasis. In this study, we evaluated hypoglycemic effect of A. formosanus extracts (AFEs) in an inducible hyperglycemia animal model and its capacity of free-radical scavenging to establish hyperglycemia-related carcinogenesis. AFE reduced blood glucose in hyperglycemic mice while there was no change in control group. The incremental area under blood glucose response curve was decreased significantly in hyperglycemic mice treated with AFE in a dose-dependent manner. AFE and metformin at the same administrated dose of 50 mg/kg showed similar effect on intraperitoneal glucose tolerance test in hyperglycemic mice. Free-radical scavenger capacity of AFE was concentration dependent and 200 μg/ml of AFE was able to reduce more than 41{\%} of the free radical. Treatment of cancer cells with AFE inhibited constitutive PD-L1 expression and its protein accumulation. It also induced expression of pro-apoptotic genes but inhibited proliferative and metastatic genes. In addition, it induced anti-proliferation in cancer cells. The results suggested that AFE not only reduced blood glucose concentration as metformin but also showed its potential use in cancer immune chemoprevention/therapy via hypoglycemic effect, ROS scavenging and PD-L1 suppression.",
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AU - Wang, Li Hsuan

AU - Hsu, Kuang Yang

AU - Chin, Yu Tang

AU - Yang, Yu Chen S.H.

AU - Wang, Shwu Huey

AU - Chen, Yi Ru

AU - Shih, Ya Jung

AU - Liu, Leroy F.

AU - Wang, Kuan

AU - Whang-Peng, Jacqueline

AU - Tang, Heng Yuan

AU - Lin, Hung Yun

AU - Liu, Hsuan Liang

AU - Lin, Shwu Jiuan

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AB - Traditional herb medicine, golden thread (Anoectochilus formosanus Hayata) has been used to treat various diseases. Hyperglycemia induces generation of reactive oxygen species (ROS) and enhancement of oxidative stress which are risk factors for cancer progression and metastasis. In this study, we evaluated hypoglycemic effect of A. formosanus extracts (AFEs) in an inducible hyperglycemia animal model and its capacity of free-radical scavenging to establish hyperglycemia-related carcinogenesis. AFE reduced blood glucose in hyperglycemic mice while there was no change in control group. The incremental area under blood glucose response curve was decreased significantly in hyperglycemic mice treated with AFE in a dose-dependent manner. AFE and metformin at the same administrated dose of 50 mg/kg showed similar effect on intraperitoneal glucose tolerance test in hyperglycemic mice. Free-radical scavenger capacity of AFE was concentration dependent and 200 μg/ml of AFE was able to reduce more than 41% of the free radical. Treatment of cancer cells with AFE inhibited constitutive PD-L1 expression and its protein accumulation. It also induced expression of pro-apoptotic genes but inhibited proliferative and metastatic genes. In addition, it induced anti-proliferation in cancer cells. The results suggested that AFE not only reduced blood glucose concentration as metformin but also showed its potential use in cancer immune chemoprevention/therapy via hypoglycemic effect, ROS scavenging and PD-L1 suppression.

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