Inhibition of xanthine oxidase by benzothiazinone analogues

Shiow Yunn Sheu, Chun Yu Lin, Jen Der Wu, Hsüch Ching Chiang

研究成果: 雜誌貢獻文章

9 引文 (Scopus)

摘要

Fourteen synthetic benzothiazinone analogues were tested for their inhibitory effects on xanthine oxidase. The enzyme, xanthine oxidase (XO) catalyses the oxidation of hypoxanthine to xanthine and of xanthine to uric acid, which has a λmax of 295 nm, forming the basis for a spectrophotometric assay for the activity of xanthine oxidase. The results showed that 2-amino-4H-1,3-benzothiazine-4-one (1), 2-guanidino-4H-1,3-benzothiazin-4-one (2) and rhodanine (3) display inhibitory effects on xanthine oxidase with an order of activity of IC-50 = 5.54, 5.60 and 121.40 μM respectively. The apparent inhibition constants (Ki) were 5.12, 19.47 and 206.23 μM, and induced non-competitive, mixed type (non-competitive-uncompetitive) and mixed type (non-competitive-uncompetitive) inhibitions respectively with respect to the substrate xanthine.

原文英語
頁(從 - 到)119-123
頁數5
期刊Anticancer Research
19
發行號1 A
出版狀態已發佈 - 1999

指紋

Xanthine Oxidase
Xanthine
Rhodanine
Hypoxanthine
Uric Acid
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

引用此文

Inhibition of xanthine oxidase by benzothiazinone analogues. / Sheu, Shiow Yunn; Lin, Chun Yu; Wu, Jen Der; Chiang, Hsüch Ching.

於: Anticancer Research, 卷 19, 編號 1 A, 1999, p. 119-123.

研究成果: 雜誌貢獻文章

Sheu, SY, Lin, CY, Wu, JD & Chiang, HC 1999, 'Inhibition of xanthine oxidase by benzothiazinone analogues', Anticancer Research, 卷 19, 編號 1 A, 頁 119-123.
Sheu, Shiow Yunn ; Lin, Chun Yu ; Wu, Jen Der ; Chiang, Hsüch Ching. / Inhibition of xanthine oxidase by benzothiazinone analogues. 於: Anticancer Research. 1999 ; 卷 19, 編號 1 A. 頁 119-123.
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