Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells

Jiun Han Chen, Junn Liang Chang, Pei Ru Chen, Yun Ju Chuang, Shih Tsang Tang, Shwu Fen Pan, Tzer Bin Lin, Kang Hua Chen, Mei Jung Chen

研究成果: 雜誌貢獻文章

5 引文 斯高帕斯(Scopus)

摘要

The purpose of this study was to investigate if PPAR γ plays a role in the melanogenesis. B16/F10 cells were divided into five groups: control, melanin stimulating hormone (-MSH), -MSH+retinol, -MSH+GW9662 (PPAR γ antagonist), and GW9662. Cells in the control group were cultured in the Dulbecco's modified Eagle's medium (DMEM) for 48 hrs. To initiate the melanogenesis, cells in all -MSH groups were cultured in medium containing -MSH (10 nM) for 48 hrs. Cells were treated simultaneously with retinol (5 M) in the -MSH+retinol group. Instead of retinol, GW9662 (10 M) was cocultured in the -MSH+GW9662 group. Cells in the final group were cultured in the DMEM with GW9662. All the analyses were carried out 48 hours after treatments. The -MSH was able to increase cell number, melanin production, and the activity of tyrosinase, the limiting enzyme in melanogenesis. These -MSH-induced changes were prevented either by retinol or by GW9662. Further analyses of the activities of antioxidant enzymes including glutathione, catalase, and the superoxide dismutase (SOD) showed that -MSH treatment raised the activity of SOD which was dependent on PPAR γ level. According to our results, the -MSH-induced melanogenesis was PPAR γ dependent, which also modulated the expression of SOD.

原文英語
文章編號695797
期刊BioMed Research International
2014
DOIs
出版狀態已發佈 - 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

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    Chen, J. H., Chang, J. L., Chen, P. R., Chuang, Y. J., Tang, S. T., Pan, S. F., Lin, T. B., Chen, K. H., & Chen, M. J. (2014). Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells. BioMed Research International, 2014, [695797]. https://doi.org/10.1155/2014/695797