Influence of silver and titanium dioxide nanoparticles on in vitro blood-brain barrier permeability

I. Chieh Chen, I. Lun Hsiao, Ho Chen Lin, Chien Hou Wu, Chun Yu Chuang, Yuh Jeen Huang

研究成果: 雜誌貢獻文章

23 引文 (Scopus)

摘要

An in vitro blood-brain barrier (BBB) model being composed of co-culture with endothelial (bEnd.3) and astrocyte-like (ALT) cells was established to evaluate the toxicity and permeability of Ag nanoparticles (AgNPs; 8 nm) and TiO2 nanoparticles (TiO2NPs; 6 nm and 35 nm) in normal and inflammatory central nervous system. Lipopolysaccharide (LPS) was pre-treated to simulate the inflammatory responses. Both AgNPs and Ag ions can decrease transendothelial electrical resistance (TEER) value, and cause discontinuous tight junction proteins (claudin-5 and zonula occludens-1) of BBB. However, only the Ag ions induced inflammatory cytokines to release, and had less cell-to-cell permeability than AgNPs, which indicated that the toxicity of AgNPs was distinct from Ag ions. LPS itself disrupted BBB, while co-treatment with AgNPs and LPS dramatically enhanced the disruption and permeability coefficient. On the other hand, TiO2NPs exposure increased BBB penetration by size, and disrupted tight junction proteins without size dependence, and many of TiO2NPs accumulated in the endothelial cells were observed. This study provided the new insight of toxic potency of AgNPs and TiO2NPs in BBB.
原文英語
頁(從 - 到)108-118
頁數11
期刊Environmental Toxicology and Pharmacology
47
DOIs
出版狀態已發佈 - 十月 1 2016
對外發佈Yes

指紋

Blood-Brain Barrier
Silver
Nanoparticles
Permeability
Tight Junction Proteins
Lipopolysaccharides
Ions
Toxicity
Claudin-5
Acoustic impedance
Tight Junctions
Poisons
Endothelial cells
Hydraulic conductivity
Neurology
Coculture Techniques
Electric Impedance
Astrocytes
Central Nervous System
Endothelial Cells

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

引用此文

Influence of silver and titanium dioxide nanoparticles on in vitro blood-brain barrier permeability. / Chen, I. Chieh; Hsiao, I. Lun; Lin, Ho Chen; Wu, Chien Hou; Chuang, Chun Yu; Huang, Yuh Jeen.

於: Environmental Toxicology and Pharmacology, 卷 47, 01.10.2016, p. 108-118.

研究成果: 雜誌貢獻文章

Chen, I. Chieh ; Hsiao, I. Lun ; Lin, Ho Chen ; Wu, Chien Hou ; Chuang, Chun Yu ; Huang, Yuh Jeen. / Influence of silver and titanium dioxide nanoparticles on in vitro blood-brain barrier permeability. 於: Environmental Toxicology and Pharmacology. 2016 ; 卷 47. 頁 108-118.
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