Induction of vasorelaxation through activation of nitric oxide synthase in endothelial cells by brazilin

Chien Ming Hu, Jaw Jou Kang, Chen Chen Lee, Ching Hao Li, Jiunn Wang Liao, Yu Wen Cheng

研究成果: 雜誌貢獻文章同行評審

55 引文 斯高帕斯(Scopus)

摘要

The vasorelaxant activity of Caesalpinia sappan L., a traditional Chinese medicine, and its major component brazilin were investigated in isolated rat aorta and human umbilical vein endothelial cells. In isolated rat aorta, C. sappan L. extract and brazilin relaxed phenylephrine-induced vasocontraction and increased cyclic guanosine 3′,5′-monophosphate (cGMP) content. Induction of vasorelaxation of brazilin was endothelium-dependent and could be markedly blocked by pretreatment with nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine methyl ester (L-NAME); NG-monomethyl-L-arginine acetate (L-NMMA) and guanylyl cyclase inhibitor, methylene blue; 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and nitric oxide (NO) scavenger, hemoglobin. The increasing cGMP content induced by brazilin was also blocked by pretreatment with L-NAME, methylene blue, and the removal of extracellular Ca2+. In human umbilical vein endothelial cells, brazilin dose-dependently induced an increase in NO formation and NOS activity, which were greatly attenuated by either the removal of extracellular Ca2+ or the chelating of intracellular Ca2+ chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA-AM). Moreover, brazilin dose-dependently induced the influx of extracellular Ca2+ in human umbilical vein endothelial cells. Collectively, these results suggest that brazilin induces vasorelaxation by the increasing intracellular Ca2+ concentration in endothelial cells of blood vessels and hence activating Ca2+/calmodulin-dependent NO synthesis. The NO is released and then transferred into smooth muscle cells to activate guanylyl cyclase and increase cGMP content, resulting in vasorelaxation.
原文英語
頁(從 - 到)37-45
頁數9
期刊European Journal of Pharmacology
468
發行號1
DOIs
出版狀態已發佈 - 五月 2 2003
對外發佈Yes

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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