Induction of skeletal muscle contracture and calcium release from isolated sarcoplasmic reticulum vesicles by sanguinarine

C. M. Hu, H. W. Cheng, Y. W. Cheng, J. J. Kang

研究成果: 雜誌貢獻文章同行評審

16 引文 斯高帕斯(Scopus)

摘要

1. The benzophenanthrine alkaloid, sanguinarine, was studied for its effects on isolated mouse phrenic-nerve diaphragm preparations. Sanguinarine induced direct, dose-dependent effects on muscle contractility. 2. Sanguinarine-induced contracture was partially inhibited when the extracellular Ca 2+ was removed or when the diaphragm was pretreated with nifedipine. Depletion of sarcoplasmic reticulum (SR) internal calcium stores completely blocked the contracture. 3. Sanguinarine induced Ca 2+ release from the actively loaded SR vesicles was blocked by ruthenium red and dithiothreitol (DTT), consistent with the ryanodine receptor (RyR) as the site of sanguinarine action. 4. Sanguinarine altered [ 3H]-ryanodine binding to the RyR of isolated SR vesicles, potentiating [ 3H]-ryanodine binding at lower concentrations and inhibiting binding at higher concentrations. All of these effects were reversed by DTT, suggesting that sanguinarine-induced Ca 2+ release from SR occurs through oxidation of critical SH groups of the RyR SR calcium release channel.
原文英語
頁(從 - 到)299-306
頁數8
期刊British Journal of Pharmacology
130
發行號2
DOIs
出版狀態已發佈 - 2000

ASJC Scopus subject areas

  • Pharmacology

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