The effects of saikosaponins (a, b1, b2, c, d), isolated from Bupleurum Radix, on the induction of differentiation in rat C6 glioma cells were studied. Saikosaponins a and d were shown to inhibit cell proliferation and alter cell morphology. In addition to cytostasis, the enzymatic activities of glutamine synthetase (GS) and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) were also noticeably increased after treatment with saikosaponin a. Nevertheless, saikosaponin d only showed an increase of GS activity, no significant changes in CNP activity were found. These results suggest that saikosaponin a can induce the differentiation of C6 glioma cells into astrocytes and/or oligodendrocytes, but saikosaponin d can only induce the differentiation of C6 glioma cells into astrocytes.
ASJC Scopus subject areas
- Organic Chemistry
- Drug Discovery
Tsai, Y. J., Chen, I. L., Horng, L. Y., & Wu, R. T. (2002). Induction of differentiation in rat C6 glioma cells with saikosaponins. Phytotherapy Research, 16(2), 117-121. https://doi.org/10.1002/ptr.752