Induction of cyclooxygenase-2 protein by lipoteichoic acid from Staphylococcus aureus in human pulmonary epithelial cells: Involvement of a nuclear factor-κB-dependent pathway

Chien-Huang Lin, I. Hui Kuan, Horng Mo Lee, Wen Sen Lee, Joen Rong Sheu, Yuan Soon Ho, Chun Hua Wang, Han Pin Kuo

研究成果: 雜誌貢獻文章

51 引文 斯高帕斯(Scopus)

摘要

1. This study investigated the role of protein kinase C (PKC) and transcription factor nuclear factor-κB (NF-κB) in cyclooxygenase-2 (COX-2) expression caused by lipoteichoic acid (LTA), a cell wall component of the gram-positive bacterium Staphylococcus aureus, in human pulmonary epithelial cell line (A549). 2. LTA caused dose- and time-dependent increases in COX-2 expression and COX activity, and a dose-dependent increase in PGE 2 release in A549 cells. The LTA-induced increases in COX-2 expression and COX activity were markedly inhibited by dexamethasone, actinomycin D or cyclohexamide, but not by polymyxin B, which binds and inactivates endotoxin. 3. The phosphatidylcholine-phospholipase C (PC-PLC) inhibitor (D-609) and the phosphatidate phosphohydrolase inhibitor (propranolol) reduced the LTA-induced increases in COX-2 expression and COX activity, while phosphatidylinositol-phospholipase C inhibitor (U-73122) had no effect. The PKC inhibitors (Go 6976, Ro 31-8220 and GF 109203X) and NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC), also attenuated the LTA-induced increases in COX-2 expression and COX activity. 4. Treatment of A549 cells with LTA caused an increase in PKC activity in the plasma membrane; this stimulatory effect was inhibited by D-609, propranolol, or Go 6976, but not by U-73122. 5. Exposure of A549 cells to LTA caused a translocation of p65 NF-κB from the cytosol to the nucleus and a degradation of IκB-α in the cytosol. Treatment of A549 cells with LTA caused NF-κB activation by detecting the formation of NF-κB-specific DNA-protein complex in the nucleus; this effect was inhibited by dexamethasone, D-609, propranolol, Go 6976, Ro 31-8220, or PDTC. 6. These results suggest that LTA might activate PC-PLC and phosphatidylcholine-phospholipase D to induce PKC activation, which in turn initiates NF-κB activation, and finally induces COX-2 expression and PGE 2 release in human pulmonary epithelial cell line.
原文英語
頁(從 - 到)543-552
頁數10
期刊British Journal of Pharmacology
134
發行號3
出版狀態已發佈 - 2001

ASJC Scopus subject areas

  • Pharmacology

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