Induction of arachidonate 12-lipoxygenase mRNA by epidermal growth factor in A431 cells

Wen Chang Chang, Yi Wen Liu, Chung Chu Ning, Hiroshi Suzuki, Tanihiro Yoshimoto, Shozo Yamamoto

研究成果: 雜誌貢獻文章

56 引文 (Scopus)

摘要

12(S)-Hydroxyeicosatetraenoic acid is biosynthesized from arachidonic acid by the microsomal fraction of human epidermoid carcinoma A431 cells, and the microsomal 12-lipoxygenase activity is enhanced by about 2-fold by epidermal growth factor (EGF) with a 10-h lag period (Chang, W. C., Ning, C. C., Lin, M. T., and Huang, J. D. (1992) J. Biol. Chem. 267, 3657-3666). The microsomal 12-lipoxygenase in A431 cells was only 3% active with linoleic acid as compared with arachidonic acid. The enzyme was immunoprecipitated by a monoclonal antibody against human platelet 12-lipoxygenase but not by that against porcine leukocyte enzyme. A 3.1-kilobase mRNA was detected in A431 cells by Northern blot analyses using cDNA probe of human platelet 12-lipoxygenase. EGF could increase the 12-lipoxygenase mRNA level by about 2-fold with a lag period of 10 h, which was well parallel with the increase in the enzyme activity. The induction of the 12-lipoxygenase mRNA by EGF was completely blocked by 35 μM cycloheximide, if present in culture medium during EGF treatment, indicating that a de novo protein biosynthesis was essential for EGF-induced 12-lipoxygenase mRNA expression. Our data provide the first evidence for the inducibility of human 12-lipoxygenase gene expression by a growth factor.

原文英語
頁(從 - 到)18734-18739
頁數6
期刊Journal of Biological Chemistry
268
發行號25
出版狀態已發佈 - 九月 5 1993
對外發佈Yes

指紋

Arachidonate 12-Lipoxygenase
Epidermal Growth Factor
Messenger RNA
Platelets
Arachidonic Acid
Enzymes
Blood Platelets
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Biosynthesis
Enzyme activity
Protein Biosynthesis
Linoleic Acid
Cycloheximide
Gene expression
Northern Blotting
Culture Media
Squamous Cell Carcinoma
Intercellular Signaling Peptides and Proteins
Leukocytes
Swine

ASJC Scopus subject areas

  • Biochemistry

引用此文

Chang, W. C., Liu, Y. W., Ning, C. C., Suzuki, H., Yoshimoto, T., & Yamamoto, S. (1993). Induction of arachidonate 12-lipoxygenase mRNA by epidermal growth factor in A431 cells. Journal of Biological Chemistry, 268(25), 18734-18739.

Induction of arachidonate 12-lipoxygenase mRNA by epidermal growth factor in A431 cells. / Chang, Wen Chang; Liu, Yi Wen; Ning, Chung Chu; Suzuki, Hiroshi; Yoshimoto, Tanihiro; Yamamoto, Shozo.

於: Journal of Biological Chemistry, 卷 268, 編號 25, 05.09.1993, p. 18734-18739.

研究成果: 雜誌貢獻文章

Chang, WC, Liu, YW, Ning, CC, Suzuki, H, Yoshimoto, T & Yamamoto, S 1993, 'Induction of arachidonate 12-lipoxygenase mRNA by epidermal growth factor in A431 cells', Journal of Biological Chemistry, 卷 268, 編號 25, 頁 18734-18739.
Chang WC, Liu YW, Ning CC, Suzuki H, Yoshimoto T, Yamamoto S. Induction of arachidonate 12-lipoxygenase mRNA by epidermal growth factor in A431 cells. Journal of Biological Chemistry. 1993 9月 5;268(25):18734-18739.
Chang, Wen Chang ; Liu, Yi Wen ; Ning, Chung Chu ; Suzuki, Hiroshi ; Yoshimoto, Tanihiro ; Yamamoto, Shozo. / Induction of arachidonate 12-lipoxygenase mRNA by epidermal growth factor in A431 cells. 於: Journal of Biological Chemistry. 1993 ; 卷 268, 編號 25. 頁 18734-18739.
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abstract = "12(S)-Hydroxyeicosatetraenoic acid is biosynthesized from arachidonic acid by the microsomal fraction of human epidermoid carcinoma A431 cells, and the microsomal 12-lipoxygenase activity is enhanced by about 2-fold by epidermal growth factor (EGF) with a 10-h lag period (Chang, W. C., Ning, C. C., Lin, M. T., and Huang, J. D. (1992) J. Biol. Chem. 267, 3657-3666). The microsomal 12-lipoxygenase in A431 cells was only 3{\%} active with linoleic acid as compared with arachidonic acid. The enzyme was immunoprecipitated by a monoclonal antibody against human platelet 12-lipoxygenase but not by that against porcine leukocyte enzyme. A 3.1-kilobase mRNA was detected in A431 cells by Northern blot analyses using cDNA probe of human platelet 12-lipoxygenase. EGF could increase the 12-lipoxygenase mRNA level by about 2-fold with a lag period of 10 h, which was well parallel with the increase in the enzyme activity. The induction of the 12-lipoxygenase mRNA by EGF was completely blocked by 35 μM cycloheximide, if present in culture medium during EGF treatment, indicating that a de novo protein biosynthesis was essential for EGF-induced 12-lipoxygenase mRNA expression. Our data provide the first evidence for the inducibility of human 12-lipoxygenase gene expression by a growth factor.",
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